
A new generation of Ca2+ indicators with greatly improved fluorescence properties.
Morgan
- 0
A new generation of Ca2+ indicators with greatly improved fluorescence properties.
A new family of highly fluorescent indicators has been synthesized for biochemical studies of the physiological role of cytosolic free Ca2+. The compounds combine an 8-coordinate tetracarboxylate chelating site with stilbene chromophores. Incorporation of the ethylenic linkage of the stilbene into a heterocyclic ring enhances the quantum efficiency and photochemical stability of the fluorophore.
Compared to their widely used predecessor, “quin2”, the new dyes offer up to 30-fold brighter fluorescence, major changes in wavelength not just intensity upon Ca2+ binding, slightly lower affinities for Ca2+, slightly longer wavelengths of excitation, and considerably improved selectivity for Ca2+ over other divalent cations. These properties, particularly the wavelength sensitivity to Ca2+, should make these dyes the preferred fluorescent indicators for many intracellular applications, especially in single cells, adherent cell layers, or bulk tissues.

TopHat: discovering splice junctions with RNA-Seq.
BACKGROUND
A new protocol for sequencing the messenger RNA in a cell, known as RNA-Seq, generates millions of short sequence fragments in a single run. These fragments, or ‘reads’, can be used to measure levels of gene expression and to identify novel splice variants of genes. However, current software for aligning RNA-Seq data to a genome relies on known splice junctions and cannot identify novel ones. TopHat is an efficient read-mapping algorithm designed to align reads from an RNA-Seq experiment to a reference genome without relying on known splice sites.
RESULTS
We mapped the RNA-Seq reads from a recent mammalian RNA-Seq experiment and recovered more than 72% of the splice junctions reported by the annotation-based software from that study, along with nearly 20,000 previously unreported junctions. The TopHat pipeline is much faster than previous systems, mapping nearly 2.2 million reads per CPU hour, which is sufficient to process an entire RNA-Seq experiment in less than a day on a standard desktop computer. We describe several challenges unique to ab initio splice site discovery from RNA-Seq reads that will require further algorithm development.
Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation.
High-throughput mRNA sequencing (RNA-Seq) promises simultaneous transcript discovery and abundance estimation. However, this would require algorithms that are not restricted by prior gene annotations and that account for alternative transcription and splicing. Here we introduce such algorithms in an open-source software program called Cufflinks. To test Cufflinks, we sequenced and analyzed >430 million paired 75-bp RNA-Seq reads from a mouse myoblast cell line over a differentiation time series. We detected 13,692 known transcripts and 3,724 previously unannotated ones, 62% of which are supported by independent expression data or by homologous genes in other species.
Over the time series, 330 genes showed complete switches in the dominant transcription start site (TSS) or splice isoform, and we observed more subtle shifts in 1,304 other genes. These results suggest that Cufflinks can illuminate the substantial regulatory flexibility and complexity in even this well-studied model of muscle development and that it can improve transcriptome-based genome annotation.
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction. The enzyme, isolated from Thermus aquaticus, greatly simplifies the procedure and, by enabling the amplification reaction to be performed at higher temperatures, significantly improves the specificity, yield, sensitivity, and length of products that can be amplified. Single-copy genomic sequences were amplified by a factor of more than 10 million with very high specificity, and DNA segments up to 2000 base pairs were readily amplified. In addition, the method was used to amplify and detect a target DNA molecule present only once in a sample of 10(5) cells.
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of extraction and assay have been optimized for maximum activity using the major late promoter of adenovirus 2. The extract also directs accurate transcription initiation from other adenovirus promoters and cellular promoters. The extract also directs accurate transcription initiation from class III promoters (tRNA and Ad 2 VA).
Molecular portraits of human breast tumours.
Human breast tumours are diverse in their natural history and in their responsiveness to treatments. Variation in transcriptional programs accounts for much of the biological diversity of human cells and tumours. In each cell, signal transduction and regulatory systems transduce information from the cell‘s identity to its environmental status, thereby controlling the level of expression of every gene in the genome. Here we have characterized variation in gene expression patterns in a set of 65 surgical specimens of human breast tumours from 42 different individuals, using complementary DNA microarrays representing 8,102 human genes. These patterns provided a distinctive molecular portrait of each tumour.
Twenty of the tumours were sampled twice, before and after a 16-week course of doxorubicin chemotherapy, and two tumours were paired with a lymph node metastasis from the same patient. Gene expression patterns in two tumour samples from the same individual were almost always more similar to each other than either was to any other sample. Sets of co-expressed genes were identified for which variation in messenger RNA levels could be related to specific features of physiological variation. The tumours could be classified into subtypes distinguished by pervasive differences in their gene expression patterns.
MUC16 Antibody |
|||
49090-100ul | SAB | 100ul | EUR 399.6 |
MUC16 Antibody |
|||
49090-50ul | SAB | 50ul | EUR 286.8 |
MUC16 antibody |
|||
70R-32038 | Fitzgerald | 100 ug | EUR 294 |
Description: Rabbit polyclonal MUC16 antibody |
MUC16 antibody |
|||
70R-15295 | Fitzgerald | 100 ug | EUR 302 |
Description: Rabbit polyclonal MUC16 antibody |
MUC16 Antibody |
|||
ABD7989 | Lifescience Market | 100 ug | EUR 525.6 |
MUC16 Antibody |
|||
1-CSB-PA268943 | Cusabio |
|
|
Description: A polyclonal antibody against MUC16. Recognizes MUC16 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:2000, IHC:1:15-1:50 |
MUC16 Antibody |
|||
DF7989 | Affbiotech | 200ul | EUR 420 |
MUC16 Antibody |
|||
DF7989-100ul | Affinity Biosciences | 100ul | EUR 168 |
Description: WB,IHC,IF/ICC,ELISA(peptide) |
MUC16 Antibody |
|||
DF7989-200ul | Affinity Biosciences | 200ul | EUR 210 |
Description: WB,IHC,IF/ICC,ELISA(peptide) |
MUC16 Antibody |
|||
1-CSB-PA015219GA01HU | Cusabio |
|
|
Description: A polyclonal antibody against MUC16. Recognizes MUC16 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB |
MUC16 Antibody |
|||
CAC07541-100ug | Biomatik Corporation | 100ug | EUR 314 |
MUC16 Antibody |
|||
CAC07541-50ug | Biomatik Corporation | 50ug | EUR 199.2 |
MUC16 Antibody |
|||
1-CSB-PA070303 | Cusabio |
|
|
Description: A polyclonal antibody against MUC16. Recognizes MUC16 from Human. This antibody is Unconjugated. Tested in the following application: IF, ELISA;IF:1/200-1/1000.ELISA:1/20000 |
MUC16 Antibody |
|||
1-CSB-PA10657A0Rb | Cusabio |
|
|
Description: A polyclonal antibody against MUC16. Recognizes MUC16 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200 |
MUC16 Antibody |
|||
CSB-PA899266- | Cusabio | each | EUR 402 |
Description: A polyclonal antibody against MUC16. Recognizes MUC16 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IF;IF:1:100-1:500 |
MUC16 Antibody |
|||
CSB-PA899266-100ul | Cusabio | 100ul | EUR 379.2 |
Description: A polyclonal antibody against MUC16. Recognizes MUC16 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IF;IF:1:100-1:500 |
MUC16 Antibody |
|||
GWB-E6791E | GenWay Biotech | 0.5 ml | Ask for price |
MUC16 Antibody |
|||
E037173 | EnoGene | 100μg/100μl | EUR 255 |
Description: Available in various conjugation types. |
MUC16 Antibody |
|||
E11-11416C | EnoGene | 100μg | EUR 225 |
Description: Available in various conjugation types. |
MUC16 Antibody |
|||
E033999 | EnoGene | 100μg/100μl | EUR 255 |
Description: Available in various conjugation types. |
MUC16 Antibody |
|||
E38PA9722 | EnoGene | 100ul | EUR 225 |
Description: Available in various conjugation types. |
MUC16 Antibody |
|||
E38PA9791 | EnoGene | 100ul | EUR 225 |
Description: Available in various conjugation types. |
MUC16 Antibody |
|||
E19-7989-1 | EnoGene | 50ug/50ul | EUR 145 |
Description: Available in various conjugation types. |
MUC16 Antibody |
|||
E19-7989-2 | EnoGene | 100ug/100ul | EUR 225 |
Description: Available in various conjugation types. |
MUC16 Antibody |
|||
MBS7130927-01mL | MyBiosource | 0.1mL | EUR 270 |
MUC16 Antibody |
|||
MBS7130927-5x01mL | MyBiosource | 5x0.1mL | EUR 1200 |
MUC16 Antibody |
|||
MBS7128000-005mL | MyBiosource | 0.05mL | EUR 190 |
MUC16 Antibody |
|||
MBS7128000-01mL | MyBiosource | 0.1mL | EUR 270 |
MUC16 Antibody |
|||
MBS7128000-5x01mL | MyBiosource | 5x0.1mL | EUR 1205 |
MUC16 Antibody |
|||
MBS7121285-005mg | MyBiosource | 0.05mg | EUR 150 |
MUC16 Antibody |
|||
MBS7121285-01mg | MyBiosource | 0.1mg | EUR 190 |
MUC16 Antibody |
|||
MBS7121285-5x01mg | MyBiosource | 5x0.1mg | EUR 845 |
MUC16 Antibody |
|||
MBS7136805-005mL | MyBiosource | 0.05mL | EUR 220 |
MUC16 Antibody |
|||
MBS7136805-01mL | MyBiosource | 0.1mL | EUR 300 |
MUC16 Antibody |
|||
MBS7136805-5x01mL | MyBiosource | 5x0.1mL | EUR 1350 |
MUC16 Antibody |
|||
MBS7136836-005mL | MyBiosource | 0.05mL | EUR 220 |
MUC16 Antibody |
|||
MBS7136836-01mL | MyBiosource | 0.1mL | EUR 300 |
MUC16 Antibody |
|||
MBS7136836-5x01mL | MyBiosource | 5x0.1mL | EUR 1350 |
MUC16 Antibody |
|||
MBS8584523-01mL | MyBiosource | 0.1mL | EUR 305 |
MUC16 Antibody |
|||
MBS8584523-01mLAF405L | MyBiosource | 0.1mL(AF405L) | EUR 465 |
MUC16 Antibody |
|||
MBS8584523-01mLAF405S | MyBiosource | 0.1mL(AF405S) | EUR 465 |
MUC16 Antibody |
|||
MBS8584523-01mLAF610 | MyBiosource | 0.1mL(AF610) | EUR 465 |
MUC16 Antibody |
|||
MBS8584523-01mLAF635 | MyBiosource | 0.1mL(AF635) | EUR 465 |
MUC16 Antibody |
|||
MBS8584592-01mL | MyBiosource | 0.1mL | EUR 305 |
MUC16 Antibody |
|||
MBS8584592-01mLAF405L | MyBiosource | 0.1mL(AF405L) | EUR 465 |
MUC16 Antibody |
|||
MBS8584592-01mLAF405S | MyBiosource | 0.1mL(AF405S) | EUR 465 |
MUC16 Antibody |
|||
MBS8584592-01mLAF610 | MyBiosource | 0.1mL(AF610) | EUR 465 |
MUC16 Antibody |
|||
MBS8584592-01mLAF635 | MyBiosource | 0.1mL(AF635) | EUR 465 |
MUC16 Antibody |
|||
MBS9608483-01mL | MyBiosource | 0.1mL | EUR 260 |
MUC16 Antibody |
|||
MBS9608483-02mL | MyBiosource | 0.2mL | EUR 305 |
MUC16 Antibody |
|||
MBS9608483-5x02mL | MyBiosource | 5x0.2mL | EUR 1220 |
MUC16 Antibody |
|||
MBS9232726-01mL | MyBiosource | 0.1mL | EUR 415 |
MUC16 Antibody |
|||
MBS9232726-5x01mL | MyBiosource | 5x0.1mL | EUR 1841 |
MUC16 Antibody |
|||
MBS8517282-005mg | MyBiosource | 0.05mg | EUR 235 |
MUC16 Antibody |
|||
MBS8517282-01mg | MyBiosource | 0.1mg | EUR 305 |
MUC16 Antibody |
|||
MBS8517282-01mLAF405M | MyBiosource | 0.1mL(AF405M) | EUR 465 |
MUC16 Antibody |
|||
MBS8517282-01mLAF546 | MyBiosource | 0.1mL(AF546) | EUR 465 |
MUC16 Antibody |
|||
MBS8517282-01mLAF750 | MyBiosource | 0.1mL(AF750) | EUR 465 |
MUC16 Antibody |
|||
MBS856468-01mg | MyBiosource | 0.1mg | EUR 305 |
MUC16 Antibody |
|||
MBS856468-01mLAF405L | MyBiosource | 0.1mL(AF405L) | EUR 465 |
MUC16 Antibody |
|||
MBS856468-01mLAF405S | MyBiosource | 0.1mL(AF405S) | EUR 465 |
MUC16 Antibody |
|||
MBS856468-01mLAF610 | MyBiosource | 0.1mL(AF610) | EUR 465 |
MUC16 Antibody |
|||
MBS856468-01mLAF635 | MyBiosource | 0.1mL(AF635) | EUR 465 |
MUC16 Antibody |
|||
MBS9404879-01mL | MyBiosource | 0.1mL | EUR 305 |
MUC16 Antibody |
|||
MBS9404879-5x01mL | MyBiosource | 5x0.1mL | EUR 1230 |
MUC16 Antibody |
|||
MBS9505374-005mL | MyBiosource | 0.05mL | EUR 285 |
MUC16 Antibody |
|||
MBS9505374-01mL | MyBiosource | 0.1mL | EUR 385 |
MUC16 Antibody |
|||
MBS9505374-5x01mL | MyBiosource | 5x0.1mL | EUR 1590 |
MUC16 Antibody |
|||
MBS9411443-005mL | MyBiosource | 0.05mL | EUR 245 |
MUC16 Antibody |
|||
MBS9411443-01mL | MyBiosource | 0.1mL | EUR 305 |
MUC16 Antibody |
|||
MBS9411443-5x01mL | MyBiosource | 5x0.1mL | EUR 1230 |
MUC16 Antibody |
|||
MBS9435863-005mL | MyBiosource | 0.05mL | EUR 325 |
MUC16 Antibody |
|||
MBS9435863-01mL | MyBiosource | 0.1mL | EUR 435 |
MUC16 Antibody |
|||
MBS9435863-5x01mL | MyBiosource | 5x0.1mL | EUR 1810 |
MUC16 Antibody |
|||
MBS9435864-005mL | MyBiosource | 0.05mL | EUR 325 |
MUC16 Antibody |
|||
MBS9435864-01mL | MyBiosource | 0.1mL | EUR 435 |
MUC16 Antibody |
|||
MBS9435864-5x01mL | MyBiosource | 5x0.1mL | EUR 1810 |
MUC16 antibody (HRP) |
|||
60R-1720 | Fitzgerald | 100 ug | EUR 302 |
Description: Rabbit polyclonal MUC16 antibody (HRP) |
MUC16 antibody (HRP) |
|||
MBS5310359-01mg | MyBiosource | 0.1mg | EUR 510 |
MUC16 antibody (HRP) |
|||
MBS5310359-5x01mg | MyBiosource | 5x0.1mg | EUR 2150 |
MUC16 antibody (FITC) |
|||
60R-1721 | Fitzgerald | 100 ug | EUR 302 |
Description: Rabbit polyclonal MUC16 antibody (FITC) |
MUC16 antibody (FITC) |
|||
MBS5310360-01mg | MyBiosource | 0.1mg | EUR 510 |
MUC16 antibody (FITC) |
|||
MBS5310360-5x01mg | MyBiosource | 5x0.1mg | EUR 2150 |
MUC16 antibody (biotin) |
|||
60R-1722 | Fitzgerald | 100 ug | EUR 302 |
Description: Rabbit polyclonal MUC16 antibody (biotin) |
MUC16 antibody (biotin) |
|||
MBS5310361-01mg | MyBiosource | 0.1mg | EUR 510 |
MUC16 antibody (biotin) |
|||
MBS5310361-5x01mg | MyBiosource | 5x0.1mg | EUR 2150 |
MUC16,CA125 Antibody |
|||
abx235429-100g | Abbexa | 100 µg | EUR 350 |
MUC16,CA125 Antibody |
|||
abx235429-100ug | Abbexa | 100 ug | EUR 661.2 |
MUC16,CA125 Antibody |
|||
abx235430-100g | Abbexa | 100 µg | EUR 350 |
MUC16,CA125 Antibody |
|||
abx235430-100ug | Abbexa | 100 ug | EUR 610.8 |
MUC16 Polyclonal Antibody |
|||
A54125 | EpiGentek |
|
|
MUC16 Polyclonal Antibody |
|||
A71345 | EpiGentek |
|
|
MUC16 polyclonal antibody |
|||
BS90892 | Bioworld Biotech | 50ul | EUR 398 |
Description: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
MUC16 polyclonal antibody |
|||
BS90893 | Bioworld Biotech | 50ul | EUR 398 |
Description: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
MUC16 Conjugated Antibody |
|||
C37173 | SAB | 100ul | EUR 476.4 |
MUC16 Conjugated Antibody |
|||
C49089 | SAB | 100ul | EUR 476.4 |
MUC16 polyclonal antibody |
|||
BS74315 | Bioworld Biotech | 50ul | EUR 358 |
Description: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
MUC16 Monoclonal Antibody |
|||
E10-31941P | EnoGene | 100μg/100μl | EUR 225 |
Description: Available in various conjugation types. |
MUC16 monoclonal antibody |
|||
MB66176 | Bioworld Biotech | 50ul | EUR 275 |
Description: Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. |
MUC16 Polyclonal Antibody |
|||
E910005 | EnoGene | 100ul | EUR 225 |
Description: Available in various conjugation types. |
MUC16 monoclonal antibody |
|||
MB66107 | Bioworld Biotech | 50ul | EUR 275 |
Description: Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. |
MUC16 Polyclonal Antibody |
|||
MBS2524578-002mL | MyBiosource | 0.02mL | EUR 140 |
MUC16 Polyclonal Antibody |
|||
MBS2524578-006mL | MyBiosource | 0.06mL | EUR 180 |
MUC16 Polyclonal Antibody |
|||
MBS2524578-012mL | MyBiosource | 0.12mL | EUR 260 |
MUC16 Polyclonal Antibody |
|||
MBS2524578-02mL | MyBiosource | 0.2mL | EUR 405 |
MUC16 Polyclonal Antibody |
|||
MBS9129130-002mL | MyBiosource | 0.02mL | EUR 200 |
MUC16 Polyclonal Antibody |
|||
MBS9129130-005mL | MyBiosource | 0.05mL | EUR 255 |
MUC16 Polyclonal Antibody |
|||
MBS9129130-01mL | MyBiosource | 0.1mL | EUR 345 |
MUC16 Polyclonal Antibody |
|||
MBS9129130-02mL | MyBiosource | 0.2mL | EUR 545 |
MUC16 Polyclonal Antibody |
|||
MBS9129130-5x02mL | MyBiosource | 5x0.2mL | EUR 2265 |
MUC16 Polyclonal Antibody |
|||
MBS8566211-01mL | MyBiosource | 0.1mL | EUR 305 |
MUC16 Polyclonal Antibody |
|||
MBS8566211-01mLAF405L | MyBiosource | 0.1mL(AF405L) | EUR 565 |
MUC16 Polyclonal Antibody |
|||
MBS8566211-01mLAF405S | MyBiosource | 0.1mL(AF405S) | EUR 565 |
MUC16 Polyclonal Antibody |
|||
MBS8566211-01mLAF610 | MyBiosource | 0.1mL(AF610) | EUR 565 |
MUC16 Polyclonal Antibody |
|||
MBS8566211-01mLAF635 | MyBiosource | 0.1mL(AF635) | EUR 565 |
MUC16 Conjugated Antibody |
|||
MBS9447616-01mLAF350 | MyBiosource | 0.1mL(AF350) | EUR 480 |
MUC16 Conjugated Antibody |
|||
MBS9447616-01mLAF405 | MyBiosource | 0.1mL(AF405) | EUR 480 |
MUC16 Conjugated Antibody |
|||
MBS9447616-01mLAF488 | MyBiosource | 0.1mL(AF488) | EUR 480 |
MUC16 Conjugated Antibody |
|||
MBS9447616-01mLAF555 | MyBiosource | 0.1mL(AF555) | EUR 480 |
MUC16 Conjugated Antibody |
|||
MBS9447616-01mLBiotin | MyBiosource | 0.1mL(Biotin) | EUR 480 |
MUC16 Conjugated Antibody |
|||
MBS9456791-01mLAF350 | MyBiosource | 0.1mL(AF350) | EUR 480 |
MUC16 Conjugated Antibody |
|||
MBS9456791-01mLAF405 | MyBiosource | 0.1mL(AF405) | EUR 480 |
MUC16 Conjugated Antibody |
|||
MBS9456791-01mLAF488 | MyBiosource | 0.1mL(AF488) | EUR 480 |
MUC16 Conjugated Antibody |
|||
MBS9456791-01mLAF555 | MyBiosource | 0.1mL(AF555) | EUR 480 |
MUC16 Conjugated Antibody |
|||
MBS9456791-01mLBiotin | MyBiosource | 0.1mL(Biotin) | EUR 480 |
×