• June 18, 2021
diavacs

Dendritic cells and the control of immunity.

Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Experimental introduction of RNA into cells can be used in certain biological systems to interfere with the function of an endogenous gene. Such effects have been proposed to result from a simple antisense mechanism that depends on hybridization between the injected RNA and endogenous messenger RNA…

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diavacs

Inflammation and cancer.

Studies on transformation of Escherichia coli with plasmids. Factors that affect the probability of genetic transformation of Escherichia coli by plasmids have been evaluated. A set of conditions is described under which about one in every 400 plasmid molecules produces a transformed cell. These conditions include cell growth in medium containing elevated levels of Mg2+, and incubation of…

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diavacs

A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. 1. The extracellular patch clamp method, which first allowed the detection of single channel currents in biological membranes, has been further refined to enable higher current resolution, direct membrane patch potential control, and physical isolation of membrane patches. 2. A description of a convenient method…

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diavacs

Transformation of intact yeast cells treated with alkali cations.

Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. The considerable therapeutic potential of human multipotent mesenchymal stromal cells (MSC) has generated markedly increasing interest in a wide variety of biomedical disciplines. However, investigators report studies of MSC using different methods of isolation and expansion, and different approaches to characterizing the cells.…

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