A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. 1. The extracellular patch clamp method, which first allowed the detection of single channel currents in biological membranes, has been further refined to enable higher current resolution, direct membrane patch potential control, and physical isolation of membrane patches. 2. A description of a convenient method…Read More