• June 25, 2021
diavacs

A new generation of Ca2+ indicators with greatly improved fluorescence properties.

A new generation of Ca2+ indicators with greatly improved fluorescence properties. A new family of highly fluorescent indicators has been synthesized for biochemical studies of the physiological role of cytosolic free Ca2+. The compounds combine an 8-coordinate tetracarboxylate chelating site with stilbene chromophores. Incorporation of the ethylenic linkage of the stilbene into a heterocyclic ring…

Read More
diavacs

Dendritic cells and the control of immunity.

Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Experimental introduction of RNA into cells can be used in certain biological systems to interfere with the function of an endogenous gene. Such effects have been proposed to result from a simple antisense mechanism that depends on hybridization between the injected RNA and endogenous messenger RNA…

Read More
diavacs

Transformation of intact yeast cells treated with alkali cations.

Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. The considerable therapeutic potential of human multipotent mesenchymal stromal cells (MSC) has generated markedly increasing interest in a wide variety of biomedical disciplines. However, investigators report studies of MSC using different methods of isolation and expansion, and different approaches to characterizing the cells.…

Read More