• May 26, 2021
diavacs

A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.

1. The extracellular patch clamp method, which first allowed the detection of single channel currents in biological membranes, has been further refined to enable higher current resolution, direct membrane patch potential control, and physical isolation of membrane patches. 2. A description of a convenient method for the fabrication of patch recording pipettes is given together with procedures followed to achieve giga-seals i.e. pipette-membrane seals with resistances of 10(9) – 10(11) omega.
3.The basic patch clamp recording circuit, and designs for improved frequency response are described along with the present limitations in recording the currents from single channels. 4. Procedures for preparation and recording from three representative cell types are given. Some properties of single acetylcholine-activated channels in muscle membrane are described to illustrate the improved current and time resolution achieved with giga-seals. 5. A description is given of the various ways that patches of membrane can be physically isolated from cells.
This isolation enables the recording of single channel currents with well-defined solutions on both sides of the membrane. Two types of isolated cell-free patch configurations can be formed: an inside-out patch with its cytoplasmic membrane face exposed to the bath solution, and an outside-out patch with its extracellular membrane face exposed to the bath solution. 6. The application of the method for the recording of ionic currents and internal dialysis of small cells is considered. Single channel resolution can be achieved when recording from whole cells, if the cell diameter is small (less than 20 micrometer). 7. The wide range of cell types amenable to giga-seal formation is discussed.
diavacs
diavacs

 

Rabbit Polyclonal antibody Anti-CRBN
Anti-CRBN 50 µg
EUR 349
ViraSafe Universal Lentiviral Expression System, Pantropic
VPK-211-PAN 1 kit
EUR 1163
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. The Pantropic Expression Systems assemble VSVG-pseudotyped lentiviruses which can easily infect virtually any cells regardless of their species of origin.
ViraSafe Lentiviral Expression System (Puro), Pantropic
VPK-212-PAN 1 kit
EUR 1163
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. The Pantropic Expression Systems assemble VSVG-pseudotyped lentiviruses which can easily infect virtually any cells regardless of their species of origin.
ViraSafe Lentiviral Expression System (Neo), Pantropic
VPK-213-PAN 1 kit
EUR 1163
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. The Pantropic Expression Systems assemble VSVG-pseudotyped lentiviruses which can easily infect virtually any cells regardless of their species of origin.
ViraSafe Lentiviral Expression System (Hygro), Pantropic
VPK-214-PAN 1 kit
EUR 1163
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. The Pantropic Expression Systems assemble VSVG-pseudotyped lentiviruses which can easily infect virtually any cells regardless of their species of origin.
ViraSafe Lentiviral Bicistronic Expression System (Puro), Pantropic
VPK-215-PAN 1 kit
EUR 1163
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. The Pantropic Expression Systems assemble VSVG-pseudotyped lentiviruses which can easily infect virtually any cells regardless of their species of origin.
ViraSafe Lentiviral Bicistronic Expression System (Neo), Pantropic
VPK-216-PAN 1 kit
EUR 1163
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. The Pantropic Expression Systems assemble VSVG-pseudotyped lentiviruses which can easily infect virtually any cells regardless of their species of origin.
ViraSafe Lentiviral Bicistronic Expression System (Hygro), Pantropic
VPK-217-PAN 1 kit
EUR 1163
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. The Pantropic Expression Systems assemble VSVG-pseudotyped lentiviruses which can easily infect virtually any cells regardless of their species of origin.
ViraSafe Lentiviral Bicistronic Expression System (Blasticidin), Pantropic
VPK-219-PAN 1 kit
EUR 1163
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. The Pantropic Expression Systems assemble VSVG-pseudotyped lentiviruses which can easily infect virtually any cells regardless of their species of origin.
Anti-CD106 / VCAM1 (Activated Endothelial Cell Marker) Monoclonal Antibody
M01199 100ug/vial
EUR 397
Description: Mouse Monoclonal CD106 / VCAM1 (Activated Endothelial Cell Marker) Antibody. Validated in IHC and tested in Human.
Anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal Antibody
M01513 100ug/vial
EUR 397
Description: Mouse Monoclonal CD31 / PECAM-1 (Endothelial Cell Marker) Antibody. Validated in Flow Cytometry, IF, IHC, WB and tested in Human, Monkey, Rabbit.
Rat Anti-Mouse Pan-endothelial Cell Antigen (MECA-32) Monoclonal antibody, clone MECA-32
CABT-L4470-1mg 1 mg
EUR 897
Rat Anti-Mouse Pan-endothelial Cell Antigen (MECA-32) Monoclonal antibody, clone MECA-32
CABT-L4470-5mg 5 mg
EUR 2405
Cytokeratin, pan (Epithelial Marker); Clone PAN-CK (Concentrate)
RA0430-C.5 0.5 ml
EUR 300
Pan-Ras Activation Assay Kit, Trial Size
STA-400-T 5 assays
EUR 403
Description: Our Ras Activation Assays use visible agarose beads to selectively precipitate the active form of specific Ras protein of interest. The precipitated small GTPase is then detected by Western blot using a target-specific antibody included in the kit. Assays are available to detect specific isoforms H-Ras, K-Ras, and N-Ras, as well as a Pan-Ras assay that detects all three isoforms.
Mouse Anti Endothelial Cell Antibody ELISA kit
E03A0434-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A sandwich ELISA for quantitative measurement of Mouse Anti Endothelial Cell Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Anti Endothelial Cell Antibody ELISA kit
E03A0434-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A sandwich ELISA for quantitative measurement of Mouse Anti Endothelial Cell Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Anti Endothelial Cell Antibody ELISA kit
E03A0434-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A sandwich ELISA for quantitative measurement of Mouse Anti Endothelial Cell Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
anti-pan-Synuclein
LF-PA0194 100 ul
EUR 334
Description: Rabbit polyclonal to pan-Synuclein
Monoclonal Cytokeratin, pan (Epithelial Marker) Antibody, Clone: PAN-CK (Cocktail)
APR14636G 7 ml
EUR 484
Description: A Monoclonal antibody against Human Cytokeratin, pan (Epithelial Marker). The antibodies are raised in Mouse and are from clone PAN-CK (Cocktail). This antibody is applicable in WB, IHC and IF, FC
Anti-Guinea pig pan T-cell antibody
STJ16100972 1 mg
EUR 562
CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody
BNUM1250-50 50uL
EUR 395
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12), 1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody
BNUM1591-50 50uL
EUR 395
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591), 1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody
BNUM1782-50 50uL
EUR 395
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782), 1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody
BNCR2332-250 250uL
EUR 394
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), RPE conjugate, Concentration: 0.1mg/mL
CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody
BNUB1250-100 100uL
EUR 209
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12), Concentration: 0.2mg/mL
CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody
BNUB1250-500 500uL
EUR 458
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12), Concentration: 0.2mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody
BNUB1591-100 100uL
EUR 209
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591), Concentration: 0.2mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody
BNUB1591-500 500uL
EUR 458
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591), Concentration: 0.2mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody
BNUB1782-100 100uL
EUR 209
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782), Concentration: 0.2mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody
BNUB1782-50 50uL
EUR 405
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782), 1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody
BNUB1782-500 500uL
EUR 458
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782), Concentration: 0.2mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody
BNUB2332-100 100uL
EUR 264
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), Concentration: 0.2mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody
BNUB2332-50 50uL
EUR 405
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), 1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody
BNUB2332-500 500uL
EUR 513
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), Concentration: 0.2mg/mL
CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody
BNC551250-100 100uL
EUR 199
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),CF555 conjugate, Concentration: 0.1mg/mL
CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody
BNC551250-500 500uL
EUR 544
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),CF555 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody
BNC551591-100 100uL
EUR 199
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF555 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody
BNC551591-500 500uL
EUR 544
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF555 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody
BNC551782-100 100uL
EUR 199
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF555 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody
BNC551782-500 500uL
EUR 544
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF555 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody
BNC552332-100 100uL
EUR 233
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF555 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody
BNC552332-500 500uL
EUR 545
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF555 conjugate, Concentration: 0.1mg/mL
CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody
BNC611250-100 100uL
EUR 199
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),CF660R conjugate, Concentration: 0.1mg/mL
CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody
BNC611250-500 500uL
EUR 544
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),CF660R conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody
BNC611591-100 100uL
EUR 199
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF660R conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody
BNC611591-500 500uL
EUR 544
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF660R conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody
BNC611782-100 100uL
EUR 199
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF660R conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody
BNC611782-500 500uL
EUR 544
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF660R conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody
BNC432332-100 100uL
EUR 233
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF543 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody
BNC432332-500 500uL
EUR 545
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF543 conjugate, Concentration: 0.1mg/mL
CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody
BNC471250-100 100uL
EUR 199
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),CF647 conjugate, Concentration: 0.1mg/mL
CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody
BNC471250-500 500uL
EUR 544
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),CF647 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody
BNC471591-100 100uL
EUR 199
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF647 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody
BNC471591-500 500uL
EUR 544
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF647 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody
BNC471782-100 100uL
EUR 199
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF647 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody
BNC471782-500 500uL
EUR 544
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF647 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody
BNC472332-100 100uL
EUR 233
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF647 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody
BNC472332-500 500uL
EUR 545
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF647 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody
BNC042332-100 100uL
EUR 233
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF405S conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody
BNC042332-500 500uL
EUR 545
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF405S conjugate, Concentration: 0.1mg/mL
CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody
BNC051250-100 100uL
EUR 199
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),CF405M conjugate, Concentration: 0.1mg/mL
CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody
BNC051250-500 500uL
EUR 544
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),CF405M conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody
BNC051591-100 100uL
EUR 199
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF405M conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody
BNC051591-500 500uL
EUR 544
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF405M conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody
BNC051782-100 100uL
EUR 199
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF405M conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody
BNC051782-500 500uL
EUR 544
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF405M conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody
BNC052332-100 100uL
EUR 233
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF405M conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody
BNC052332-500 500uL
EUR 545
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF405M conjugate, Concentration: 0.1mg/mL
CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody
BNC401250-100 100uL
EUR 199
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),CF640R conjugate, Concentration: 0.1mg/mL
CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody
BNC401250-500 500uL
EUR 544
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),CF640R conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody
BNC401591-100 100uL
EUR 199
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF640R conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody
BNC401591-500 500uL
EUR 544
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF640R conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody
BNC401782-100 100uL
EUR 199
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF640R conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody
BNC401782-500 500uL
EUR 544
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF640R conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody
BNC402332-100 100uL
EUR 233
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF640R conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody
BNC402332-500 500uL
EUR 545
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF640R conjugate, Concentration: 0.1mg/mL
CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody
BNC431250-100 100uL
EUR 199
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),CF543 conjugate, Concentration: 0.1mg/mL
CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody
BNC431250-500 500uL
EUR 544
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),CF543 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody
BNC431591-100 100uL
EUR 199
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF543 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody
BNC431591-500 500uL
EUR 544
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF543 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody
BNC431782-100 100uL
EUR 199
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF543 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody
BNC431782-500 500uL
EUR 544
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF543 conjugate, Concentration: 0.1mg/mL
CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody
BNC041250-100 100uL
EUR 199
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),CF405S conjugate, Concentration: 0.1mg/mL
CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody
BNC041250-500 500uL
EUR 544
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),CF405S conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody
BNC041591-100 100uL
EUR 199
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF405S conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody
BNC041591-500 500uL
EUR 544
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF405S conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody
BNC041782-100 100uL
EUR 199
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF405S conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody
BNC041782-500 500uL
EUR 544
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF405S conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody
BNC701591-100 100uL
EUR 199
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF770 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody
BNC701591-500 500uL
EUR 544
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF770 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody
BNC701782-100 100uL
EUR 199
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF770 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody
BNC701782-500 500uL
EUR 544
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF770 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody
BNC702332-100 100uL
EUR 233
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF770 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody
BNC702332-500 500uL
EUR 545
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF770 conjugate, Concentration: 0.1mg/mL
CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody
BNC801250-100 100uL
EUR 199
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),CF680 conjugate, Concentration: 0.1mg/mL
CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody
BNC801250-500 500uL
EUR 544
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),CF680 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody
BNC801591-100 100uL
EUR 199
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF680 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody
BNC801591-500 500uL
EUR 544
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF680 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody
BNC801782-100 100uL
EUR 199
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF680 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody
BNC801782-500 500uL
EUR 544
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF680 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody
BNC802332-100 100uL
EUR 233
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF680 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody
BNC802332-500 500uL
EUR 545
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF680 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody
BNCH1591-100 100uL
EUR 199
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody
BNCH1591-500 500uL
EUR 544
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody
BNCH1782-100 100uL
EUR 199
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody
BNCH1782-500 500uL
EUR 544
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody
BNCH2332-100 100uL
EUR 233
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody
BNCH2332-500 500uL
EUR 545
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL
CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody
BNCP1250-250 250uL
EUR 383
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),PerCP conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody
BNCP1591-250 250uL
EUR 383
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),PerCP conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody
BNCP1782-250 250uL
EUR 383
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),PerCP conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody
BNCP2332-250 250uL
EUR 394
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), PerCP conjugate, Concentration: 0.1mg/mL
CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody
BNCR1250-250 250uL
EUR 383
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),RPE conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody
BNCR1591-250 250uL
EUR 383
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),RPE conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody
BNCR1782-250 250uL
EUR 383
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),RPE conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody
BNC941591-100 100uL
EUR 199
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF594 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody
BNC941591-500 500uL
EUR 544
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF594 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody
BNC941782-100 100uL
EUR 199
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF594 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody
BNC941782-500 500uL
EUR 544
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF594 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody
BNC942332-100 100uL
EUR 233
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF594 conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody
BNC942332-500 500uL
EUR 545
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF594 conjugate, Concentration: 0.1mg/mL
CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody
BNCA1250-250 250uL
EUR 383
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),APC conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody
BNCA1591-250 250uL
EUR 383
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),APC conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody
BNCA1782-250 250uL
EUR 383
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),APC conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody
BNCA2332-250 250uL
EUR 394
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), APC conjugate, Concentration: 0.1mg/mL
CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody
BNCB1250-100 100uL
EUR 199
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),Biotin conjugate, Concentration: 0.1mg/mL
CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody
BNCB1250-500 500uL
EUR 544
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),Biotin conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody
BNCB1591-100 100uL
EUR 199
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),Biotin conjugate, Concentration: 0.1mg/mL
Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody
BNCB1591-500 500uL
EUR 544
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),Biotin conjugate, Concentration: 0.1mg/mL

A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

A procedure for extracting plasmid DNA from bacterial cells is described. The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes. The principle of the method is selective alkaline denaturation of high molecular weight chromosomal DNA while covalently closed circular DNA remains double-stranded. Adequate pH control is accomplished without using a pH meter. Upon neutralization, chromosomal DNA renatures to form an insoluble clot, leaving plasmid DNA in the supernatant. Large and small plasmid DNAs have been extracted by this method.

KEGG: kyoto encyclopedia of genes and genomes.

KEGG (Kyoto Encyclopedia of Genes and Genomes) is a knowledge base for systematic analysis of gene functions, linking genomic information with higher order functional information. The genomic information is stored in the GENES database, which is a collection of gene catalogs for all the completely sequenced genomes and some partial genomes with up-to-date annotation of gene functions. The higher order functional information is stored in the PATHWAY database, which contains graphical representations of cellular processes, such as metabolism, membrane transport, signal transduction and cell cycle.
The PATHWAY database is supplemented by a set of ortholog group tables for the information about conserved subpathways (pathway motifs), which are often encoded by positionally coupled genes on the chromosome and which are especially useful in predicting gene functions. A third database in KEGG is LIGAND for the information about chemical compounds, enzyme molecules and enzymatic reactions. KEGG provides Java graphics tools for browsing genome maps, comparing two genome maps and manipulating expression maps, as well as computational tools for sequence comparison, graph comparison and path computation. The KEGG databases are daily updated and made freely available (http://www. genome.ad.jp/kegg/).

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