• May 26, 2021
diavacs

A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.

1. The extracellular patch clamp method, which first allowed the detection of single channel currents in biological membranes, has been further refined to enable higher current resolution, direct membrane patch potential control, and physical isolation of membrane patches. 2. A description of a convenient method for the fabrication of patch recording pipettes is given together with procedures followed to achieve giga-seals i.e. pipette-membrane seals with resistances of 10(9) – 10(11) omega.
3.The basic patch clamp recording circuit, and designs for improved frequency response are described along with the present limitations in recording the currents from single channels. 4. Procedures for preparation and recording from three representative cell types are given. Some properties of single acetylcholine-activated channels in muscle membrane are described to illustrate the improved current and time resolution achieved with giga-seals. 5. A description is given of the various ways that patches of membrane can be physically isolated from cells.
This isolation enables the recording of single channel currents with well-defined solutions on both sides of the membrane. Two types of isolated cell-free patch configurations can be formed: an inside-out patch with its cytoplasmic membrane face exposed to the bath solution, and an outside-out patch with its extracellular membrane face exposed to the bath solution. 6. The application of the method for the recording of ionic currents and internal dialysis of small cells is considered. Single channel resolution can be achieved when recording from whole cells, if the cell diameter is small (less than 20 micrometer). 7. The wide range of cell types amenable to giga-seal formation is discussed.
diavacs
diavacs

 

RECA1 (Pan Endothelial Marker) (FITC)

MBS6250184-01mL 0.1(mL
EUR 875

RECA1 (Pan Endothelial Marker) (FITC)

MBS6250184-5x01mL 5x0.1mL
EUR 3800

RECA1 (Pan Endothelial Marker) (Biotin)

MBS6249277-01mL 0.1(mL
EUR 875

RECA1 (Pan Endothelial Marker) (Biotin)

MBS6249277-5x01mL 5x0.1mL
EUR 3800

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438463-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438463-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438463-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438463-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438463-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438490-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438490-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438490-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438490-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438490-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438528-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438528-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438528-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438528-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438528-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438712-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438712-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438712-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438712-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438712-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438783-05mL 0.5mL
EUR 405

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438783-5x05mL 5x0.5mL
EUR 1725

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS439142-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS439142-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS439142-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS439142-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS439142-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS439291-05mL 0.5mL
EUR 405

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS439291-5x05mL 5x0.5mL
EUR 1725

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS439540-01mL 0.1mL
EUR 230

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS439540-05mL 0.5mL
EUR 405

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS439540-5x01mL 5x0.1mL
EUR 925

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS439598-05mL 0.5mL
EUR 405

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS439598-5x05mL 5x0.5mL
EUR 1725

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438010-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438010-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438010-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438010-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438010-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438221-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438221-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438221-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438221-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438221-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438300-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438300-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438300-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438300-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438300-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

RECA1 (Pan Endothelial Marker) (MaxLight 490)

MBS6254217-01mL 0.1(mL
EUR 875

RECA1 (Pan Endothelial Marker) (MaxLight 490)

MBS6254217-5x01mL 5x0.1mL
EUR 3800

RECA1 (Pan Endothelial Marker) (MaxLight 550)

MBS6255257-01mL 0.1(mL
EUR 875

RECA1 (Pan Endothelial Marker) (MaxLight 550)

MBS6255257-5x01mL 5x0.1mL
EUR 3800

RECA1 (Pan Endothelial Marker) (MaxLight 405)

MBS6253177-01mL 0.1(mL
EUR 875

RECA1 (Pan Endothelial Marker) (MaxLight 405)

MBS6253177-5x01mL 5x0.1mL
EUR 3800

RECA1 (Pan Endothelial Marker) (MaxLight 750)

MBS6257336-01mL 0.1mL
EUR 875

RECA1 (Pan Endothelial Marker) (MaxLight 750)

MBS6257336-5x01mL 5x0.1mL
EUR 3800

RECA1 (Pan Endothelial Marker) (MaxLight 650)

MBS6256297-01mL 0.1(mL
EUR 875

RECA1 (Pan Endothelial Marker) (MaxLight 650)

MBS6256297-5x01mL 5x0.1mL
EUR 3800

CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438774-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438774-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438774-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438774-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438774-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438966-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438966-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438966-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438966-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS438966-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS439086-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS439086-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS439086-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS439086-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody

MBS439086-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4380148-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4380148-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4380148-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4380148-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4380148-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4380153-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4380153-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4380153-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4380153-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4380153-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4380652-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4380652-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4380652-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4380652-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4380652-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381154-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381154-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381154-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381154-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381154-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381155-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381155-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381155-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381155-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381155-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381156-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381156-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381156-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381156-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381156-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381157-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381157-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381157-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381157-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381157-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381158-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381158-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381158-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381158-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381158-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381159-05mL 0.5mL
EUR 405

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381159-5x05mL 5x0.5mL
EUR 1725

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381160-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381160-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381160-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381160-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381160-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381161-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381161-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381161-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381161-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

CD31/PECAM-1 (Endothelial Cell Marker)

MBS4381161-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

A procedure for extracting plasmid DNA from bacterial cells is described. The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes. The principle of the method is selective alkaline denaturation of high molecular weight chromosomal DNA while covalently closed circular DNA remains double-stranded. Adequate pH control is accomplished without using a pH meter. Upon neutralization, chromosomal DNA renatures to form an insoluble clot, leaving plasmid DNA in the supernatant. Large and small plasmid DNAs have been extracted by this method.

KEGG: kyoto encyclopedia of genes and genomes.

KEGG (Kyoto Encyclopedia of Genes and Genomes) is a knowledge base for systematic analysis of gene functions, linking genomic information with higher order functional information. The genomic information is stored in the GENES database, which is a collection of gene catalogs for all the completely sequenced genomes and some partial genomes with up-to-date annotation of gene functions. The higher order functional information is stored in the PATHWAY database, which contains graphical representations of cellular processes, such as metabolism, membrane transport, signal transduction and cell cycle.
The PATHWAY database is supplemented by a set of ortholog group tables for the information about conserved subpathways (pathway motifs), which are often encoded by positionally coupled genes on the chromosome and which are especially useful in predicting gene functions. A third database in KEGG is LIGAND for the information about chemical compounds, enzyme molecules and enzymatic reactions. KEGG provides Java graphics tools for browsing genome maps, comparing two genome maps and manipulating expression maps, as well as computational tools for sequence comparison, graph comparison and path computation. The KEGG databases are daily updated and made freely available (http://www. genome.ad.jp/kegg/).

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