• May 26, 2021
diavacs

A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.

1. The extracellular patch clamp method, which first allowed the detection of single channel currents in biological membranes, has been further refined to enable higher current resolution, direct membrane patch potential control, and physical isolation of membrane patches. 2. A description of a convenient method for the fabrication of patch recording pipettes is given together with procedures followed to achieve giga-seals i.e. pipette-membrane seals with resistances of 10(9) – 10(11) omega.
3.The basic patch clamp recording circuit, and designs for improved frequency response are described along with the present limitations in recording the currents from single channels. 4. Procedures for preparation and recording from three representative cell types are given. Some properties of single acetylcholine-activated channels in muscle membrane are described to illustrate the improved current and time resolution achieved with giga-seals. 5. A description is given of the various ways that patches of membrane can be physically isolated from cells.
This isolation enables the recording of single channel currents with well-defined solutions on both sides of the membrane. Two types of isolated cell-free patch configurations can be formed: an inside-out patch with its cytoplasmic membrane face exposed to the bath solution, and an outside-out patch with its extracellular membrane face exposed to the bath solution. 6. The application of the method for the recording of ionic currents and internal dialysis of small cells is considered. Single channel resolution can be achieved when recording from whole cells, if the cell diameter is small (less than 20 micrometer). 7. The wide range of cell types amenable to giga-seal formation is discussed.
diavacs
diavacs

 

CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody

BNC811250-100 100uL
EUR 238.8
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),CF680R conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody

BNC811250-500 500uL
EUR 652.8
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),CF680R conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody

BNC551250-100 100uL
EUR 238.8
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),CF555 conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody

BNC551250-500 500uL
EUR 652.8
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),CF555 conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody

BNC431250-100 100uL
EUR 238.8
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),CF543 conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody

BNC431250-500 500uL
EUR 652.8
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),CF543 conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody

BNC611250-100 100uL
EUR 238.8
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),CF660R conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody

BNC611250-500 500uL
EUR 652.8
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),CF660R conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody

BNC051250-100 100uL
EUR 238.8
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),CF405M conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody

BNC051250-500 500uL
EUR 652.8
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),CF405M conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody

BNCA1250-250 250uL
EUR 459.6
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),APC conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody

BNCP1250-250 250uL
EUR 459.6
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),PerCP conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12) Antibody

BNCR1250-250 250uL
EUR 459.6
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12),RPE conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker)(), 1mg/mL

BNUM1662-50 1uL
EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD31 / PECAM-1 (Endothelial Cell Marker)(), 0.2mg/mL

BNUB1662-100 1uL
EUR 225
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD31 / PECAM-1 (Endothelial Cell Marker)(), 0.2mg/mL

BNUB1662-500 1uL
EUR 485
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

Anti-Thrombomodulin / CD141 (Endothelial Cell Marker) Monoclonal Antibody

M01325 100ug/vial
EUR 476.4
Description: Mouse Monoclonal Thrombomodulin / CD141 (Endothelial Cell Marker) Antibody. Validated in IHC and tested in Human.

CD34,(Endothelial Cell Marker) Antibody, Ready-To-Use

MAB430P 5ml
EUR 425

Anti-CD106 / VCAM1 (Activated Endothelial Cell Marker) Monoclonal Antibody

M01199 100ug/vial
EUR 476.4
Description: Mouse Monoclonal CD106 / VCAM1 (Activated Endothelial Cell Marker) Antibody. Validated in IHC and tested in Human.

CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R) Antibody

BNCAP1395-100 100uL
EUR 238.8
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R),Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R) Antibody

BNCAP1395-500 500uL
EUR 652.8
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R),Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R) Antibody

BNCH1395-100 100uL
EUR 238.8
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R),Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R) Antibody

BNCH1395-500 500uL
EUR 652.8
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R),Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R) Antibody

BNC701395-100 100uL
EUR 238.8
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R),CF770 conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R) Antibody

BNC701395-500 500uL
EUR 652.8
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R),CF770 conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R) Antibody

BNC801395-100 100uL
EUR 238.8
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R),CF680 conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R) Antibody

BNC801395-500 500uL
EUR 652.8
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R),CF680 conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R) Antibody

BNC811395-100 100uL
EUR 238.8
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R),CF680R conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R) Antibody

BNC811395-500 500uL
EUR 652.8
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R),CF680R conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R) Antibody

BNC551395-100 100uL
EUR 238.8
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R),CF555 conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R) Antibody

BNC551395-500 500uL
EUR 652.8
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R),CF555 conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R) Antibody

BNC611395-100 100uL
EUR 238.8
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R),CF660R conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R) Antibody

BNC611395-500 500uL
EUR 652.8
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R),CF660R conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R) Antibody

BNC431395-100 100uL
EUR 238.8
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R),CF543 conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R) Antibody

BNC431395-500 500uL
EUR 652.8
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R),CF543 conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R) Antibody

BNC051395-100 100uL
EUR 238.8
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R),CF405M conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R) Antibody

BNC051395-500 500uL
EUR 652.8
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R),CF405M conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R) Antibody

BNCA1395-250 250uL
EUR 459.6
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R),APC conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R) Antibody

BNCP1395-250 250uL
EUR 459.6
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R),PerCP conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R) Antibody

BNCR1395-250 250uL
EUR 459.6
Description: Primary antibody against CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R),RPE conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12), 1mg/mL

BNUM1250-50 50uL
EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12), 0.2mg/mL

BNUB1250-100 100uL
EUR 225
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD31 / PECAM-1 (Endothelial Cell Marker) (C31.12), 0.2mg/mL

BNUB1250-500 500uL
EUR 485
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD31 / PECAM-1 (Endothelial Cell Marker)(rC31.3), 0.2mg/mL

BNUB3462-100 1uL
EUR 225
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD31 / PECAM-1 (Endothelial Cell Marker)(rC31.3), 0.2mg/mL

BNUB3462-500 1uL
EUR 485
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD31 / PECAM-1 (Endothelial Cell Marker)(1A10), 1mg/mL

BNUM0846-50 50uL
EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD31 / PECAM-1 (Endothelial Cell Marker)(rC31.3), 1mg/mL

BNUM3462-50 1uL
EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD31 / PECAM-1 (Endothelial Cell Marker)(1A10), 0.2mg/mL

BNUB0846-100 100uL
EUR 225
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD31 / PECAM-1 (Endothelial Cell Marker)(1A10), 0.2mg/mL

BNUB0846-500 500uL
EUR 485
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody

BNUB1782-50 50uL
EUR 486
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782), 1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody

BNC701591-100 100uL
EUR 238.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF770 conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody

BNC701591-500 500uL
EUR 652.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF770 conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody

BNC701782-100 100uL
EUR 238.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF770 conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody

BNC701782-500 500uL
EUR 652.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF770 conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody

BNC801591-100 100uL
EUR 238.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF680 conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody

BNC801591-500 500uL
EUR 652.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF680 conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody

BNC801782-100 100uL
EUR 238.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF680 conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody

BNC801782-500 500uL
EUR 652.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF680 conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody

BNC811591-100 100uL
EUR 238.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF680R conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody

BNC811591-500 500uL
EUR 652.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF680R conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody

BNC811782-100 100uL
EUR 238.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF680R conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody

BNC811782-500 500uL
EUR 652.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF680R conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody

BNC551782-100 100uL
EUR 238.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF555 conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody

BNC551782-500 500uL
EUR 652.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF555 conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody

BNC611591-100 100uL
EUR 238.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF660R conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody

BNC611591-500 500uL
EUR 652.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF660R conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody

BNC611782-100 100uL
EUR 238.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF660R conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody

BNC611782-500 500uL
EUR 652.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF660R conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody

BNC431591-100 100uL
EUR 238.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF543 conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody

BNC431591-500 500uL
EUR 652.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF543 conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody

BNC431782-100 100uL
EUR 238.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF543 conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody

BNC431782-500 500uL
EUR 652.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF543 conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody

BNC051591-100 100uL
EUR 238.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF405M conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody

BNC051591-500 500uL
EUR 652.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),CF405M conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody

BNC051782-100 100uL
EUR 238.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF405M conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody

BNC051782-500 500uL
EUR 652.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),CF405M conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody

BNCA1591-250 250uL
EUR 459.6
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),APC conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody

BNCA1782-250 250uL
EUR 459.6
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),APC conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody

BNCAP1591-100 100uL
EUR 238.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody

BNCAP1591-500 500uL
EUR 652.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody

BNCAP1782-100 100uL
EUR 238.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody

BNCAP1782-500 500uL
EUR 652.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody

BNCH1591-100 100uL
EUR 238.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody

BNCH1591-500 500uL
EUR 652.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody

BNCH1782-100 100uL
EUR 238.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody

BNCH1782-500 500uL
EUR 652.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody

BNCP1591-250 250uL
EUR 459.6
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),PerCP conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody

BNCP1782-250 250uL
EUR 459.6
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),PerCP conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591) Antibody

BNCR1591-250 250uL
EUR 459.6
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591),RPE conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782) Antibody

BNCR1782-250 250uL
EUR 459.6
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1782),RPE conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody

BNUB2332-50 50uL
EUR 486
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), 1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody

BNC702332-100 100uL
EUR 279.6
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF770 conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody

BNC702332-500 500uL
EUR 654
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF770 conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody

BNC802332-100 100uL
EUR 279.6
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF680 conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody

BNC802332-500 500uL
EUR 654
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF680 conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody

BNC812332-100 100uL
EUR 279.6
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF680R conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody

BNC812332-500 500uL
EUR 654
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF680R conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody

BNC552332-100 100uL
EUR 279.6
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF555 conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody

BNC552332-500 500uL
EUR 654
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF555 conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody

BNC612332-100 100uL
EUR 279.6
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF660R conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody

BNC612332-500 500uL
EUR 654
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF660R conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody

BNC432332-100 100uL
EUR 279.6
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF543 conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody

BNC432332-500 500uL
EUR 654
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF543 conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody

BNC052332-100 100uL
EUR 279.6
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF405M conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody

BNC052332-500 500uL
EUR 654
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), CF405M conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody

BNCA2332-250 250uL
EUR 472.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), APC conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody

BNCAP2332-100 100uL
EUR 279.6
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody

BNCAP2332-500 500uL
EUR 654
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody

BNCH2332-100 100uL
EUR 279.6
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody

BNCH2332-500 500uL
EUR 654
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody

BNCP2332-250 250uL
EUR 472.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), PerCP conjugate, Concentration: 0.1mg/mL

Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591) Antibody

BNCR2332-250 250uL
EUR 472.8
Description: Primary antibody against Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), RPE conjugate, Concentration: 0.1mg/mL

CD31 / PECAM-1 (Endothelial Cell Marker)(C31/2876R), 0.2mg/mL

BNUB2876-100 1uL
EUR 225
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD31 / PECAM-1 (Endothelial Cell Marker)(C31/2876R), 0.2mg/mL

BNUB2876-500 1uL
EUR 485
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R), 1mg/mL

BNUM1395-50 50uL
EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD31 / PECAM-1 (Endothelial Cell Marker)(C31/2876R), 1mg/mL

BNUM2876-50 1uL
EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R), 0.2mg/mL

BNUB1395-100 100uL
EUR 225
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R), 0.2mg/mL

BNUB1395-500 500uL
EUR 485
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD31 / PECAM-1 (Endothelial Cell Marker)(PECAM1/3526), 0.2mg/mL

BNUB3526-100 1uL
EUR 225
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD31 / PECAM-1 (Endothelial Cell Marker)(PECAM1/3526), 0.2mg/mL

BNUB3526-500 1uL
EUR 485
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD31 / PECAM-1 (Endothelial Cell Marker)(PECAM1/3527), 0.2mg/mL

BNUB3527-100 1uL
EUR 225
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD31 / PECAM-1 (Endothelial Cell Marker)(PECAM1/3527), 0.2mg/mL

BNUB3527-500 1uL
EUR 485
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD31 / PECAM-1 (Endothelial Cell Marker)(PECAM1/3528), 0.2mg/mL

BNUB3528-100 1uL
EUR 225
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD31 / PECAM-1 (Endothelial Cell Marker)(PECAM1/3528), 0.2mg/mL

BNUB3528-500 1uL
EUR 485
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD31 / PECAM-1 (Endothelial Cell Marker)(PECAM1/3529), 0.2mg/mL

BNUB3529-100 1uL
EUR 225
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD31 / PECAM-1 (Endothelial Cell Marker)(PECAM1/3529), 0.2mg/mL

BNUB3529-500 1uL
EUR 485
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD31 / PECAM-1 (Endothelial Cell Marker)(PECAM1/3530), 0.2mg/mL

BNUB3530-100 1uL
EUR 225
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD31 / PECAM-1 (Endothelial Cell Marker)(PECAM1/3530), 0.2mg/mL

BNUB3530-500 1uL
EUR 485
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD31 / PECAM-1 (Endothelial Cell Marker)(PECAM1/3540), 0.2mg/mL

BNUB3540-100 1uL
EUR 225
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD31 / PECAM-1 (Endothelial Cell Marker)(PECAM1/3540), 0.2mg/mL

BNUB3540-500 1uL
EUR 485
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

A procedure for extracting plasmid DNA from bacterial cells is described. The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes. The principle of the method is selective alkaline denaturation of high molecular weight chromosomal DNA while covalently closed circular DNA remains double-stranded. Adequate pH control is accomplished without using a pH meter. Upon neutralization, chromosomal DNA renatures to form an insoluble clot, leaving plasmid DNA in the supernatant. Large and small plasmid DNAs have been extracted by this method.

KEGG: kyoto encyclopedia of genes and genomes.

KEGG (Kyoto Encyclopedia of Genes and Genomes) is a knowledge base for systematic analysis of gene functions, linking genomic information with higher order functional information. The genomic information is stored in the GENES database, which is a collection of gene catalogs for all the completely sequenced genomes and some partial genomes with up-to-date annotation of gene functions. The higher order functional information is stored in the PATHWAY database, which contains graphical representations of cellular processes, such as metabolism, membrane transport, signal transduction and cell cycle.
The PATHWAY database is supplemented by a set of ortholog group tables for the information about conserved subpathways (pathway motifs), which are often encoded by positionally coupled genes on the chromosome and which are especially useful in predicting gene functions. A third database in KEGG is LIGAND for the information about chemical compounds, enzyme molecules and enzymatic reactions. KEGG provides Java graphics tools for browsing genome maps, comparing two genome maps and manipulating expression maps, as well as computational tools for sequence comparison, graph comparison and path computation. The KEGG databases are daily updated and made freely available (http://www. genome.ad.jp/kegg/).

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