A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
- Morgan
- 0
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1. The extracellular patch clamp method, which first allowed the detection of single channel currents in biological membranes, has been further refined to enable higher current resolution, direct membrane patch potential control, and physical isolation of membrane patches. 2. A description of a convenient method for the fabrication of patch recording pipettes is given together with procedures followed to achieve giga-seals i.e. pipette-membrane seals with resistances of 10(9) – 10(11) omega.
3.The basic patch clamp recording circuit, and designs for improved frequency response are described along with the present limitations in recording the currents from single channels. 4. Procedures for preparation and recording from three representative cell types are given. Some properties of single acetylcholine-activated channels in muscle membrane are described to illustrate the improved current and time resolution achieved with giga-seals. 5. A description is given of the various ways that patches of membrane can be physically isolated from cells.
This isolation enables the recording of single channel currents with well-defined solutions on both sides of the membrane. Two types of isolated cell-free patch configurations can be formed: an inside-out patch with its cytoplasmic membrane face exposed to the bath solution, and an outside-out patch with its extracellular membrane face exposed to the bath solution. 6. The application of the method for the recording of ionic currents and internal dialysis of small cells is considered. Single channel resolution can be achieved when recording from whole cells, if the cell diameter is small (less than 20 micrometer). 7. The wide range of cell types amenable to giga-seal formation is discussed.
RECA1 (Pan Endothelial Marker) (FITC) |
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MBS6250184-01mL | MyBiosource | 0.1(mL | EUR 875 |
RECA1 (Pan Endothelial Marker) (FITC) |
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MBS6250184-5x01mL | MyBiosource | 5x0.1mL | EUR 3800 |
RECA1 (Pan Endothelial Marker) (Biotin) |
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MBS6249277-01mL | MyBiosource | 0.1(mL | EUR 875 |
RECA1 (Pan Endothelial Marker) (Biotin) |
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MBS6249277-5x01mL | MyBiosource | 5x0.1mL | EUR 3800 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438463-002mgWithBSAAzideat02mgmL | MyBiosource | 0.02mg(WithBSA&Azideat0.2mg/mL) | EUR 230 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438463-01mgWithBSAAzideat02mgmL | MyBiosource | 0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 405 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
|||
MBS438463-01mgWithoutBSAAzideat1mgmL | MyBiosource | 0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 405 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438463-5x01mgWithBSAAzideat02mgmL | MyBiosource | 5x0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 1725 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438463-5x01mgWithoutBSAAzideat1mgmL | MyBiosource | 5x0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 1725 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438490-002mgWithBSAAzideat02mgmL | MyBiosource | 0.02mg(WithBSA&Azideat0.2mg/mL) | EUR 230 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438490-01mgWithBSAAzideat02mgmL | MyBiosource | 0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 405 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438490-01mgWithoutBSAAzideat1mgmL | MyBiosource | 0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 405 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438490-5x01mgWithBSAAzideat02mgmL | MyBiosource | 5x0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 1725 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438490-5x01mgWithoutBSAAzideat1mgmL | MyBiosource | 5x0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 1725 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438528-002mgWithBSAAzideat02mgmL | MyBiosource | 0.02mg(WithBSA&Azideat0.2mg/mL) | EUR 230 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438528-01mgWithBSAAzideat02mgmL | MyBiosource | 0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 405 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438528-01mgWithoutBSAAzideat1mgmL | MyBiosource | 0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 405 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438528-5x01mgWithBSAAzideat02mgmL | MyBiosource | 5x0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 1725 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438528-5x01mgWithoutBSAAzideat1mgmL | MyBiosource | 5x0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 1725 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438712-002mgWithBSAAzideat02mgmL | MyBiosource | 0.02mg(WithBSA&Azideat0.2mg/mL) | EUR 230 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438712-01mgWithBSAAzideat02mgmL | MyBiosource | 0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 405 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438712-01mgWithoutBSAAzideat1mgmL | MyBiosource | 0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 405 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
|||
MBS438712-5x01mgWithBSAAzideat02mgmL | MyBiosource | 5x0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 1725 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438712-5x01mgWithoutBSAAzideat1mgmL | MyBiosource | 5x0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 1725 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438783-05mL | MyBiosource | 0.5mL | EUR 405 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438783-5x05mL | MyBiosource | 5x0.5mL | EUR 1725 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS439142-002mgWithBSAAzideat02mgmL | MyBiosource | 0.02mg(WithBSA&Azideat0.2mg/mL) | EUR 230 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS439142-01mgWithBSAAzideat02mgmL | MyBiosource | 0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 405 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS439142-01mgWithoutBSAAzideat1mgmL | MyBiosource | 0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 405 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS439142-5x01mgWithBSAAzideat02mgmL | MyBiosource | 5x0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 1725 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS439142-5x01mgWithoutBSAAzideat1mgmL | MyBiosource | 5x0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 1725 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS439291-05mL | MyBiosource | 0.5mL | EUR 405 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS439291-5x05mL | MyBiosource | 5x0.5mL | EUR 1725 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS439540-01mL | MyBiosource | 0.1mL | EUR 230 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS439540-05mL | MyBiosource | 0.5mL | EUR 405 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS439540-5x01mL | MyBiosource | 5x0.1mL | EUR 925 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS439598-05mL | MyBiosource | 0.5mL | EUR 405 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS439598-5x05mL | MyBiosource | 5x0.5mL | EUR 1725 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438010-002mgWithBSAAzideat02mgmL | MyBiosource | 0.02mg(WithBSA&Azideat0.2mg/mL) | EUR 230 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438010-01mgWithBSAAzideat02mgmL | MyBiosource | 0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 405 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438010-01mgWithoutBSAAzideat1mgmL | MyBiosource | 0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 405 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438010-5x01mgWithBSAAzideat02mgmL | MyBiosource | 5x0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 1725 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438010-5x01mgWithoutBSAAzideat1mgmL | MyBiosource | 5x0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 1725 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438221-002mgWithBSAAzideat02mgmL | MyBiosource | 0.02mg(WithBSA&Azideat0.2mg/mL) | EUR 230 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438221-01mgWithBSAAzideat02mgmL | MyBiosource | 0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 405 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438221-01mgWithoutBSAAzideat1mgmL | MyBiosource | 0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 405 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438221-5x01mgWithBSAAzideat02mgmL | MyBiosource | 5x0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 1725 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438221-5x01mgWithoutBSAAzideat1mgmL | MyBiosource | 5x0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 1725 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438300-002mgWithBSAAzideat02mgmL | MyBiosource | 0.02mg(WithBSA&Azideat0.2mg/mL) | EUR 230 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438300-01mgWithBSAAzideat02mgmL | MyBiosource | 0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 405 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438300-01mgWithoutBSAAzideat1mgmL | MyBiosource | 0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 405 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438300-5x01mgWithBSAAzideat02mgmL | MyBiosource | 5x0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 1725 |
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438300-5x01mgWithoutBSAAzideat1mgmL | MyBiosource | 5x0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 1725 |
RECA1 (Pan Endothelial Marker) (MaxLight 490) |
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MBS6254217-01mL | MyBiosource | 0.1(mL | EUR 875 |
RECA1 (Pan Endothelial Marker) (MaxLight 490) |
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MBS6254217-5x01mL | MyBiosource | 5x0.1mL | EUR 3800 |
RECA1 (Pan Endothelial Marker) (MaxLight 550) |
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MBS6255257-01mL | MyBiosource | 0.1(mL | EUR 875 |
RECA1 (Pan Endothelial Marker) (MaxLight 550) |
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MBS6255257-5x01mL | MyBiosource | 5x0.1mL | EUR 3800 |
RECA1 (Pan Endothelial Marker) (MaxLight 405) |
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MBS6253177-01mL | MyBiosource | 0.1(mL | EUR 875 |
RECA1 (Pan Endothelial Marker) (MaxLight 405) |
|||
MBS6253177-5x01mL | MyBiosource | 5x0.1mL | EUR 3800 |
RECA1 (Pan Endothelial Marker) (MaxLight 750) |
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MBS6257336-01mL | MyBiosource | 0.1mL | EUR 875 |
RECA1 (Pan Endothelial Marker) (MaxLight 750) |
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MBS6257336-5x01mL | MyBiosource | 5x0.1mL | EUR 3800 |
RECA1 (Pan Endothelial Marker) (MaxLight 650) |
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MBS6256297-01mL | MyBiosource | 0.1(mL | EUR 875 |
RECA1 (Pan Endothelial Marker) (MaxLight 650) |
|||
MBS6256297-5x01mL | MyBiosource | 5x0.1mL | EUR 3800 |
CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438774-002mgWithBSAAzideat02mgmL | MyBiosource | 0.02mg(WithBSA&Azideat0.2mg/mL) | EUR 230 |
CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438774-01mgWithBSAAzideat02mgmL | MyBiosource | 0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 405 |
CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody |
|||
MBS438774-01mgWithoutBSAAzideat1mgmL | MyBiosource | 0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 405 |
CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody |
|||
MBS438774-5x01mgWithBSAAzideat02mgmL | MyBiosource | 5x0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 1725 |
CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438774-5x01mgWithoutBSAAzideat1mgmL | MyBiosource | 5x0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 1725 |
CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438966-002mgWithBSAAzideat02mgmL | MyBiosource | 0.02mg(WithBSA&Azideat0.2mg/mL) | EUR 230 |
CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438966-01mgWithBSAAzideat02mgmL | MyBiosource | 0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 405 |
CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody |
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MBS438966-01mgWithoutBSAAzideat1mgmL | MyBiosource | 0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 405 |
CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody |
|||
MBS438966-5x01mgWithBSAAzideat02mgmL | MyBiosource | 5x0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 1725 |
CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody |
|||
MBS438966-5x01mgWithoutBSAAzideat1mgmL | MyBiosource | 5x0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 1725 |
CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody |
|||
MBS439086-002mgWithBSAAzideat02mgmL | MyBiosource | 0.02mg(WithBSA&Azideat0.2mg/mL) | EUR 230 |
CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody |
|||
MBS439086-01mgWithBSAAzideat02mgmL | MyBiosource | 0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 405 |
CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody |
|||
MBS439086-01mgWithoutBSAAzideat1mgmL | MyBiosource | 0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 405 |
CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody |
|||
MBS439086-5x01mgWithBSAAzideat02mgmL | MyBiosource | 5x0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 1725 |
CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody |
|||
MBS439086-5x01mgWithoutBSAAzideat1mgmL | MyBiosource | 5x0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 1725 |
CD31/PECAM-1 (Endothelial Cell Marker) |
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MBS4380148-002mgWithBSAAzideat02mgmL | MyBiosource | 0.02mg(WithBSA&Azideat0.2mg/mL) | EUR 230 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4380148-01mgWithBSAAzideat02mgmL | MyBiosource | 0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 405 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4380148-01mgWithoutBSAAzideat1mgmL | MyBiosource | 0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 405 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4380148-5x01mgWithBSAAzideat02mgmL | MyBiosource | 5x0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 1725 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4380148-5x01mgWithoutBSAAzideat1mgmL | MyBiosource | 5x0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 1725 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4380153-002mgWithBSAAzideat02mgmL | MyBiosource | 0.02mg(WithBSA&Azideat0.2mg/mL) | EUR 230 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4380153-01mgWithBSAAzideat02mgmL | MyBiosource | 0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 405 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4380153-01mgWithoutBSAAzideat1mgmL | MyBiosource | 0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 405 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4380153-5x01mgWithBSAAzideat02mgmL | MyBiosource | 5x0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 1725 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4380153-5x01mgWithoutBSAAzideat1mgmL | MyBiosource | 5x0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 1725 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4380652-002mgWithBSAAzideat02mgmL | MyBiosource | 0.02mg(WithBSA&Azideat0.2mg/mL) | EUR 230 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4380652-01mgWithBSAAzideat02mgmL | MyBiosource | 0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 405 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4380652-01mgWithoutBSAAzideat1mgmL | MyBiosource | 0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 405 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4380652-5x01mgWithBSAAzideat02mgmL | MyBiosource | 5x0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 1725 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4380652-5x01mgWithoutBSAAzideat1mgmL | MyBiosource | 5x0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 1725 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381154-002mgWithBSAAzideat02mgmL | MyBiosource | 0.02mg(WithBSA&Azideat0.2mg/mL) | EUR 230 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381154-01mgWithBSAAzideat02mgmL | MyBiosource | 0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 405 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381154-01mgWithoutBSAAzideat1mgmL | MyBiosource | 0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 405 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381154-5x01mgWithBSAAzideat02mgmL | MyBiosource | 5x0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 1725 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381154-5x01mgWithoutBSAAzideat1mgmL | MyBiosource | 5x0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 1725 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381155-002mgWithBSAAzideat02mgmL | MyBiosource | 0.02mg(WithBSA&Azideat0.2mg/mL) | EUR 230 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381155-01mgWithBSAAzideat02mgmL | MyBiosource | 0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 405 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381155-01mgWithoutBSAAzideat1mgmL | MyBiosource | 0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 405 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381155-5x01mgWithBSAAzideat02mgmL | MyBiosource | 5x0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 1725 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381155-5x01mgWithoutBSAAzideat1mgmL | MyBiosource | 5x0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 1725 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381156-002mgWithBSAAzideat02mgmL | MyBiosource | 0.02mg(WithBSA&Azideat0.2mg/mL) | EUR 230 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381156-01mgWithBSAAzideat02mgmL | MyBiosource | 0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 405 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381156-01mgWithoutBSAAzideat1mgmL | MyBiosource | 0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 405 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381156-5x01mgWithBSAAzideat02mgmL | MyBiosource | 5x0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 1725 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381156-5x01mgWithoutBSAAzideat1mgmL | MyBiosource | 5x0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 1725 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381157-002mgWithBSAAzideat02mgmL | MyBiosource | 0.02mg(WithBSA&Azideat0.2mg/mL) | EUR 230 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381157-01mgWithBSAAzideat02mgmL | MyBiosource | 0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 405 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381157-01mgWithoutBSAAzideat1mgmL | MyBiosource | 0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 405 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381157-5x01mgWithBSAAzideat02mgmL | MyBiosource | 5x0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 1725 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381157-5x01mgWithoutBSAAzideat1mgmL | MyBiosource | 5x0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 1725 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381158-002mgWithBSAAzideat02mgmL | MyBiosource | 0.02mg(WithBSA&Azideat0.2mg/mL) | EUR 230 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381158-01mgWithBSAAzideat02mgmL | MyBiosource | 0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 405 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381158-01mgWithoutBSAAzideat1mgmL | MyBiosource | 0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 405 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381158-5x01mgWithBSAAzideat02mgmL | MyBiosource | 5x0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 1725 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381158-5x01mgWithoutBSAAzideat1mgmL | MyBiosource | 5x0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 1725 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381159-05mL | MyBiosource | 0.5mL | EUR 405 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381159-5x05mL | MyBiosource | 5x0.5mL | EUR 1725 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381160-002mgWithBSAAzideat02mgmL | MyBiosource | 0.02mg(WithBSA&Azideat0.2mg/mL) | EUR 230 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381160-01mgWithBSAAzideat02mgmL | MyBiosource | 0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 405 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381160-01mgWithoutBSAAzideat1mgmL | MyBiosource | 0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 405 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381160-5x01mgWithBSAAzideat02mgmL | MyBiosource | 5x0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 1725 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381160-5x01mgWithoutBSAAzideat1mgmL | MyBiosource | 5x0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 1725 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381161-002mgWithBSAAzideat02mgmL | MyBiosource | 0.02mg(WithBSA&Azideat0.2mg/mL) | EUR 230 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381161-01mgWithBSAAzideat02mgmL | MyBiosource | 0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 405 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381161-01mgWithoutBSAAzideat1mgmL | MyBiosource | 0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 405 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381161-5x01mgWithBSAAzideat02mgmL | MyBiosource | 5x0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 1725 |
CD31/PECAM-1 (Endothelial Cell Marker) |
|||
MBS4381161-5x01mgWithoutBSAAzideat1mgmL | MyBiosource | 5x0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 1725 |
×
A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
A procedure for extracting plasmid DNA from bacterial cells is described. The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes. The principle of the method is selective alkaline denaturation of high molecular weight chromosomal DNA while covalently closed circular DNA remains double-stranded. Adequate pH control is accomplished without using a pH meter. Upon neutralization, chromosomal DNA renatures to form an insoluble clot, leaving plasmid DNA in the supernatant. Large and small plasmid DNAs have been extracted by this method.
KEGG: kyoto encyclopedia of genes and genomes.
KEGG (Kyoto Encyclopedia of Genes and Genomes) is a knowledge base for systematic analysis of gene functions, linking genomic information with higher order functional information. The genomic information is stored in the GENES database, which is a collection of gene catalogs for all the completely sequenced genomes and some partial genomes with up-to-date annotation of gene functions. The higher order functional information is stored in the PATHWAY database, which contains graphical representations of cellular processes, such as metabolism, membrane transport, signal transduction and cell cycle.
The PATHWAY database is supplemented by a set of ortholog group tables for the information about conserved subpathways (pathway motifs), which are often encoded by positionally coupled genes on the chromosome and which are especially useful in predicting gene functions. A third database in KEGG is LIGAND for the information about chemical compounds, enzyme molecules and enzymatic reactions. KEGG provides Java graphics tools for browsing genome maps, comparing two genome maps and manipulating expression maps, as well as computational tools for sequence comparison, graph comparison and path computation. The KEGG databases are daily updated and made freely available (http://www. genome.ad.jp/kegg/).
Induction of pluripotent stem cells from adult human fibroblasts by defined factors.
Successful reprogramming of differentiated human somatic cells into a pluripotent state would allow creation of patient- and disease-specific stem cells. We previously reported generation of induced pluripotent stem (iPS) cells, capable of germline transmission, from mouse somatic cells by transduction of four defined transcription factors.
Here, we demonstrate the generation of iPS cells from adult human dermal fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc. Human iPS cells were similar to human embryonic stem (ES) cells in morphology, proliferation, surface antigens, gene expression, epigenetic status of pluripotent cell-specific genes, and telomerase activity. Furthermore, these cells could differentiate into cell types of the three germ layers in vitro and in teratomas. These findings demonstrate that iPS cells can be generated from adult human fibroblasts.
Significance analysis of microarrays applied to the ionizing radiation response.
Microarrays can measure the expression of thousands of genes to identify changes in expression between different biological states. Methods are needed to determine the significance of these changes while accounting for the enormous number of genes. We describe a method, Significance Analysis of Microarrays (SAM), that assigns a score to each gene on the basis of change in gene expression relative to the standard deviation of repeated measurements. For genes with scores greater than an adjustable threshold, SAM uses permutations of the repeated measurements to estimate the percentage of genes identified by chance, the false discovery rate (FDR).
When the transcriptional response of human cells to ionizing radiation was measured by microarrays, SAM identified 34 genes that changed at least 1.5-fold with an estimated FDR of 12%, compared with FDRs of 60 and 84% by using conventional methods of analysis. Of the 34 genes, 19 were involved in cell cycle regulation and 3 in apoptosis. Surprisingly, four nucleotide excision repair genes were induced, suggesting that this repair pathway for UV-damaged DNA might play a previously unrecognized role in repairing DNA damaged by ionizing radiation.
rHu MIF |
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AK8255-0025 | Akron Biotech | 25µg | Ask for price |
rHu MIF |
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AK8255-0100 | Akron Biotech | 100µg | Ask for price |
rHu MIF |
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AK8255-1000 | Akron Biotech | 1mg | Ask for price |
rHu EGF |
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AK8327-0100 | Akron Biotech | 100µg | Ask for price |
rHu EGF |
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AK8327-0500 | Akron Biotech | 500µg | Ask for price |
rHu EGF |
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AK8327-1000 | Akron Biotech | 1mg | Ask for price |
rHu SHH |
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AK9088-0005 | Akron Biotech | 5µg | Ask for price |
rHu SHH |
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AK9088-0025 | Akron Biotech | 25µg | Ask for price |
rHu SHH |
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AK9088-0100 | Akron Biotech | 100µg | Ask for price |
rHu SHH |
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AK9088-1000 | Akron Biotech | 1mg | Ask for price |
rHu TPO |
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AK9089-0002 | Akron Biotech | 5µg | Ask for price |
rHu TPO |
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AK9089-0010 | Akron Biotech | 20µg | Ask for price |
rHu TPO |
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AK9089-0100 | Akron Biotech | 100µg | Ask for price |
rHu TPO |
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AK9089-1000 | Akron Biotech | 1mg | Ask for price |
rHu PTN |
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AK9912-0005 | Akron Biotech | 5µg | Ask for price |
rHu PTN |
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AK9912-0020 | Akron Biotech | 20µg | Ask for price |
rHu PTN |
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AK9912-0100 | Akron Biotech | 100µg | Ask for price |
rHu PTN |
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AK9912-1000 | Akron Biotech | 1mg | Ask for price |
rHu LIF |
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AK9977-0005 | Akron Biotech | 5µg | Ask for price |
rHu LIF |
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AK9977-0025 | Akron Biotech | 25µg | Ask for price |
rHu LIF |
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AK9977-0100 | Akron Biotech | 100µg | Ask for price |
rHu LIF |
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AK9977-1000 | Akron Biotech | 1mg | Ask for price |
rHu MIG |
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AK9979-0005 | Akron Biotech | 5µg | Ask for price |
rHu MIG |
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AK9979-0020 | Akron Biotech | 20µg | Ask for price |
rHu MIG |
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AK9979-0100 | Akron Biotech | 100µg | Ask for price |
rHu MIG |
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AK9979-1000 | Akron Biotech | 1mg | Ask for price |
rHu aFGF |
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AK8230-0010 | Akron Biotech | 10µg | Ask for price |
rHu aFGF |
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AK8230-0050 | Akron Biotech | 50µg | Ask for price |
rHu aFGF |
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AK8230-0100 | Akron Biotech | 100µg | Ask for price |
rHu aFGF |
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AK8230-1000 | Akron Biotech | 1mg | Ask for price |
rHu MCSF |
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AK8238-0002 | Akron Biotech | 2µg | Ask for price |
rHu MCSF |
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AK8238-0010 | Akron Biotech | 10µg | Ask for price |
rHu MCSF |
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AK8238-0100 | Akron Biotech | 100µg | Ask for price |
rHu MCSF |
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AK8238-1000 | Akron Biotech | 1mg | Ask for price |
rHu CTGF |
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AK8240-0005 | Akron Biotech | 5µg | Ask for price |
rHu CTGF |
|||
AK8240-0020 | Akron Biotech | 20µg | Ask for price |
rHu CTGF |
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AK8240-0100 | Akron Biotech | 100µg | Ask for price |
rHu CTGF |
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AK8240-1000 | Akron Biotech | 1mg | Ask for price |
rHu BDNF |
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AK8286-0002 | Akron Biotech | 2µg | Ask for price |
rHu BDNF |
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AK8286-0010 | Akron Biotech | 10µg | Ask for price |
rHu BDNF |
|||
AK8286-0100 | Akron Biotech | 100µg | Ask for price |
rHu BDNF |
|||
AK8286-1000 | Akron Biotech | 1mg | Ask for price |
rHu CNTF |
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AK8302-0005 | Akron Biotech | 5µg | Ask for price |
rHu CNTF |
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AK8302-0020 | Akron Biotech | 20µg | Ask for price |
rHu CNTF |
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AK8302-0100 | Akron Biotech | 100µg | Ask for price |
rHu CNTF |
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AK8302-1000 | Akron Biotech | 1mg | Ask for price |
rHu GDNF |
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AK8335-0002 | Akron Biotech | 2µg | Ask for price |
rHu GDNF |
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AK8335-0010 | Akron Biotech | 10µg | Ask for price |
rHu GDNF |
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AK8335-0100 | Akron Biotech | 100µg | Ask for price |
rHu GDNF |
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AK8335-1000 | Akron Biotech | 1mg | Ask for price |
rHu BAFF |
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AK8540-0005 | Akron Biotech | 5µg | Ask for price |
rHu BAFF |
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AK8540-0020 | Akron Biotech | 20µg | Ask for price |
rHu BAFF |
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AK8540-0100 | Akron Biotech | 100µg | Ask for price |
rHu BAFF |
|||
AK8540-1000 | Akron Biotech | 1mg | Ask for price |
rHu TSLP |
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AK9091-0002 | Akron Biotech | 2µg | Ask for price |
rHu TSLP |
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AK9091-0010 | Akron Biotech | 10µg | Ask for price |
rHu TSLP |
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AK9091-0100 | Akron Biotech | 100µg | Ask for price |
rHu TSLP |
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AK9091-1000 | Akron Biotech | 1mg | Ask for price |
rHu TRAIL |
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AK9090-0010 | Akron Biotech | 10µg | Ask for price |
rHu TRAIL |
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AK9090-0050 | Akron Biotech | 50µg | Ask for price |
rHu TRAIL |
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AK9090-0100 | Akron Biotech | 100µg | Ask for price |
rHu TRAIL |
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AK9090-1000 | Akron Biotech | 1mg | Ask for price |
rHu Leptin |
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AK8265-0200 | Akron Biotech | 200µg | Ask for price |
rHu Leptin |
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AK8265-1000 | Akron Biotech | 1mg | Ask for price |
rHu Leptin |
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AK8265-5000 | Akron Biotech | 5mg | Ask for price |
rHu RANTES |
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AK8275-0005 | Akron Biotech | 5µg | Ask for price |
rHu RANTES |
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AK8275-0020 | Akron Biotech | 20µg | Ask for price |
rHu RANTES |
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AK8275-0100 | Akron Biotech | 100µg | Ask for price |
rHu RANTES |
|||
AK8275-1000 | Akron Biotech | 1mg | Ask for price |
rHu Insulin |
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AK8211-0025 | Akron Biotech | 25mg | Ask for price |
rHu Insulin |
|||
AK8211-0250 | Akron Biotech | 250mg | Ask for price |
rHu Insulin |
|||
AK8211-1000 | Akron Biotech | 1g | Ask for price |
rHu Artemin |
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AK9076-0005 | Akron Biotech | 5µg | Ask for price |
rHu Artemin |
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AK9076-0020 | Akron Biotech | 20µg | Ask for price |
rHu Artemin |
|||
AK9076-0100 | Akron Biotech | 100µg | Ask for price |
rHu Artemin |
|||
AK9076-1000 | Akron Biotech | 1mg | Ask for price |
rHu Omentin |
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AK9083-0002 | Akron Biotech | 2µg | Ask for price |
rHu Omentin |
|||
AK9083-0010 | Akron Biotech | 10µg | Ask for price |
rHu Omentin |
|||
AK9083-0100 | Akron Biotech | 100µg | Ask for price |
rHu Omentin |
|||
AK9083-1000 | Akron Biotech | 1mg | Ask for price |
rHu Eotaxin |
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AK9969-0005 | Akron Biotech | 5µg | Ask for price |
rHu Eotaxin |
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AK9969-0020 | Akron Biotech | 20µg | Ask for price |
rHu Eotaxin |
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AK9969-0100 | Akron Biotech | 100µg | Ask for price |
rHu Eotaxin |
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AK9969-1000 | Akron Biotech | 1mg | Ask for price |
rHu Resistin |
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AK9086-0005 | Akron Biotech | 5µg | Ask for price |
rHu Resistin |
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AK9086-0025 | Akron Biotech | 25µg | Ask for price |
rHu Resistin |
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AK9086-0100 | Akron Biotech | 100µg | Ask for price |
rHu Resistin |
|||
AK9086-1000 | Akron Biotech | 1mg | Ask for price |
rHu Chemerin |
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AK9697-0005 | Akron Biotech | 5µg | Ask for price |
rHu Chemerin |
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AK9697-0025 | Akron Biotech | 25µg | Ask for price |
rHu Chemerin |
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AK9697-0100 | Akron Biotech | 100µg | Ask for price |
rHu Chemerin |
|||
AK9697-1000 | Akron Biotech | 1mg | Ask for price |
rHu Visfatin |
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AK9981-0005 | Akron Biotech | 5µg | Ask for price |
rHu Visfatin |
|||
AK9981-0025 | Akron Biotech | 25µg | Ask for price |
rHu Visfatin |
|||
AK9981-0100 | Akron Biotech | 100µg | Ask for price |
rHu Visfatin |
|||
AK9981-1000 | Akron Biotech | 1mg | Ask for price |
rHu Myostatin |
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AK8256-0002 | Akron Biotech | 2µg | Ask for price |
rHu Myostatin |
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AK8256-0010 | Akron Biotech | 10µg | Ask for price |
rHu Myostatin |
|||
AK8256-0100 | Akron Biotech | 100µg | Ask for price |
rHu Myostatin |
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AK8256-1000 | Akron Biotech | 1mg | Ask for price |
rHu Prolactin |
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AK9964-0010 | Akron Biotech | 10µg | Ask for price |
rHu Prolactin |
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AK9964-0050 | Akron Biotech | 50µg | Ask for price |
rHu Prolactin |
|||
AK9964-0100 | Akron Biotech | 100µg | Ask for price |
rHu Prolactin |
|||
AK9964-1000 | Akron Biotech | 1mg | Ask for price |
rHu Persephin |
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AK9980-0005 | Akron Biotech | 5µg | Ask for price |
rHu Persephin |
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AK9980-0020 | Akron Biotech | 20µg | Ask for price |
rHu Persephin |
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AK9980-0100 | Akron Biotech | 100µg | Ask for price |
rHu Persephin |
|||
AK9980-1000 | Akron Biotech | 1mg | Ask for price |
rHu Follistatin |
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AK9971-0005 | Akron Biotech | 5µg | Ask for price |
rHu Follistatin |
|||
AK9971-0020 | Akron Biotech | 20µg | Ask for price |
rHu Follistatin |
|||
AK9971-0100 | Akron Biotech | 100µg | Ask for price |
rHu Follistatin |
|||
AK9971-1000 | Akron Biotech | 1mg | Ask for price |
rHu Fractalkine |
|||
AK9973-0005 | Akron Biotech | 5µg | Ask for price |
rHu Fractalkine |
|||
AK9973-0020 | Akron Biotech | 20µg | Ask for price |
rHu Fractalkine |
|||
AK9973-0100 | Akron Biotech | 100µg | Ask for price |
rHu Fractalkine |
|||
AK9973-1000 | Akron Biotech | 1mg | Ask for price |
rHu Procalcitonin |
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AK9084-0002 | Akron Biotech | 2µg | Ask for price |
rHu Procalcitonin |
|||
AK9084-0010 | Akron Biotech | 10µg | Ask for price |
rHu Procalcitonin |
|||
AK9084-0100 | Akron Biotech | 100µg | Ask for price |
rHu Procalcitonin |
|||
AK9084-1000 | Akron Biotech | 1mg | Ask for price |
rHu IL-2 |
|||
AK8277-0010 | Akron Biotech | 10µg | Ask for price |
rHu IL-2 |
|||
AK8277-0050 | Akron Biotech | 50µg | Ask for price |
rHu IL-2 |
|||
AK8277-0100 | Akron Biotech | 100µg | Ask for price |
rHu IL-2 |
|||
AK8277-1000 | Akron Biotech | 1mg | Ask for price |
rHu IL-7 |
|||
AK8285-0002 | Akron Biotech | 2µg | Ask for price |
rHu IL-7 |
|||
AK8285-0010 | Akron Biotech | 10µg | Ask for price |
rHu IL-7 |
|||
AK8285-0100 | Akron Biotech | 100µg | Ask for price |
rHu IL-7 |
|||
AK8285-1000 | Akron Biotech | 1mg | Ask for price |
rHu IL-3 |
|||
AK8321-0002 | Akron Biotech | 2µg | Ask for price |
rHu IL-3 |
|||
AK8321-0010 | Akron Biotech | 10µg | Ask for price |
rHu IL-3 |
|||
AK8321-0100 | Akron Biotech | 100µg | Ask for price |
rHu IL-3 |
|||
AK8321-1000 | Akron Biotech | 1mg | Ask for price |
rHu IL-4 |
|||
AK8329-0005 | Akron Biotech | 5µg | Ask for price |
rHu IL-4 |
|||
AK8329-0020 | Akron Biotech | 20µg | Ask for price |
rHu IL-4 |
|||
AK8329-0100 | Akron Biotech | 100µg | Ask for price |
rHu IL-4 |
|||
AK8329-1000 | Akron Biotech | 1mg | Ask for price |
rHu IL-6 |
|||
AK8334-0005 | Akron Biotech | 5µg | Ask for price |
rHu IL-6 |
|||
AK8334-0020 | Akron Biotech | 20µg | Ask for price |
rHu IL-6 |
|||
AK8334-0100 | Akron Biotech | 100µg | Ask for price |
rHu IL-6 |
|||
AK8334-1000 | Akron Biotech | 1mg | Ask for price |
rHu NT-3 |
|||
AK8543-0002 | Akron Biotech | 2µg | Ask for price |
rHu NT-3 |
|||
AK8543-0010 | Akron Biotech | 10µg | Ask for price |
rHu NT-3 |
|||
AK8543-0100 | Akron Biotech | 100µg | Ask for price |
rHu NT-3 |
|||
AK8543-1000 | Akron Biotech | 1mg | Ask for price |
×