Hallmarks of cancer: the next generation.
Hallmarks of cancer: the next generation.
The hallmarks of cancer comprise six biological capabilities acquired during the multistep development of human tumors. The hallmarks constitute an organizing principle for rationalizing the complexities of neoplastic disease. They include sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, and activating invasion and metastasis. Underlying these hallmarks are genome instability, which generates the genetic diversity that expedites their acquisition, and inflammation, which fosters multiple hallmark functions.
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Conceptual progress in the last decade has added two emerging hallmarks of potential generality to this list-reprogramming of energy metabolism and evading immune destruction. In addition to cancer cells, tumors exhibit another dimension of complexity: they contain a repertoire of recruited, ostensibly normal cells that contribute to the acquisition of hallmark traits by creating the “tumor microenvironment.” Recognition of the widespread applicability of these concepts will increasingly affect the development of new means to treat human cancer.
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples.
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
Differentiated cells can be reprogrammed to an embryonic-like state by transfer of nuclear contents into oocytes or by fusion with embryonic stem (ES) cells. Little is known about factors that induce this reprogramming. Here, we demonstrate induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions. Unexpectedly, Nanog was dispensable. These cells, which we designated iPS (induced pluripotent stem) cells, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
Subcutaneous transplantation of iPS cells into nude mice resulted in tumors containing a variety of tissues from all three germ layers. Following injection into blastocysts, iPS cells contributed to mouse embryonic development. These data demonstrate that pluripotent stem cells can be directly generated from fibroblast cultures by the addition of only a few defined factors.
Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man.
The steady-state basal plasma glucose and insulin concentrations are determined by their interaction in a feedback loop. A computer-solved model has been used to predict the homeostatic concentrations which arise from varying degrees beta-cell deficiency and insulin resistance. Comparison of a patient’s fasting values with the model’s predictions allows a quantitative assessment of the contributions of insulin resistance and deficient beta-cell function to the fasting hyperglycaemia (homeostasis model assessment, HOMA). The accuracy and precision of the estimate have been determined by comparison with independent measures of insulin resistance and beta-cell function using hyperglycaemic and euglycaemic clamps and an intravenous glucose tolerance test.
The estimate of insulin resistance obtained by homeostasis model assessment correlated with estimates obtained by use of the euglycaemic clamp (Rs = 0.88, p less than 0.0001), the fasting insulin concentration (Rs = 0.81, p less than 0.0001), and the hyperglycaemic clamp, (Rs = 0.69, p less than 0.01). There was no correlation with any aspect of insulin-receptor binding. The estimate of deficient beta-cell function obtained by homeostasis model assessment correlated with that derived using the hyperglycaemic clamp (Rs = 0.61, p less than 0.01) and with the estimate from the intravenous glucose tolerance test (Rs = 0.64, p less than 0.05).
The low precision of the estimates from the model (coefficients of variation: 31% for insulin resistance and 32% for beta-cell deficit) limits its use, but the correlation of the model’s estimates with patient data accords with the hypothesis that basal glucose and insulin interactions are largely determined by a simple feed back loop.
Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.
A tetrazolium salt has been used to develop a quantitative colorimetric assay for mammalian cell survival and proliferation. The assay detects living, but not dead cells and the signal generated is dependent on the degree of activation of the cells. This method can therefore be used to measure cytotoxicity, proliferation or activation.
The results can be read on a multiwell scanning spectrophotometer (ELISA reader) and show a high degree of precision. No washing steps are used in the assay. The main advantages of the colorimetric assay are its rapidity and precision, and the lack of any radioisotope. We have used the assay to measure proliferative lymphokines, mitogen stimulations and complement-mediated lysis.
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.
The Agrobacterium vacuum infiltration method has made it possible to transform Arabidopsis thaliana without plant tissue culture or regeneration. In the present study, this method was evaluated and a substantially modified transformation method was developed. The labor-intensive vacuum infiltration process was eliminated in favor of simple dipping of developing floral tissues into a solution containing
Agrobacterium tumefaciens, 5% sucrose and 500 microliters per litre of surfactant Silwet L-77. Sucrose and surfactant were critical to the success of the floral dip method. Plants inoculated when numerous immature floral buds and few siliques were present produced transformed progeny at the highest rate. Plant tissue culture media, the hormone benzylamino purine and pH adjustment were unnecessary, and Agrobacterium could be applied to plants at a range of cell densities. Repeated application of Agrobacterium improved transformation rates and overall yield of transformants approximately twofold.
Covering plants for 1 day to retain humidity after inoculation also raised transformation rates twofold. Multiple ecotypes were transformable by this method. The modified method should facilitate high-throughput transformation of Arabidopsis for efforts such as T-DNA gene tagging, positional cloning, or attempts at targeted gene replacement.
 Betacellulin, human |
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| RC218-23 | Bio Basic | 5ug | EUR 104.38 |
 Anti-Betacellulin/Btc Antibody |
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| A02171-1 | BosterBio | 100ug/vial | EUR 334 |
 Rabbit Polyclonal antibody Anti-CRBN |
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| Anti-CRBN | ImmunoStep | 50 µg | EUR 349 |
 Recombinant Human Betacellulin |
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| 7-00223 | CHI Scientific | 5µg | Ask for price |
Recombinant Human Betacellulin |
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| 7-00224 | CHI Scientific | 20µg | Ask for price |
Recombinant Human Betacellulin |
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| 7-00225 | CHI Scientific | 1mg | Ask for price |
 Betacellulin protein |
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| 30R-AB028 | Fitzgerald | 20 ug | EUR 273 |
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Description: Purified recombinant Human Betacellulin protein |
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 Bovine Betacellulin |
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| ARU020 | GroPep | 20 µg | EUR 253 |
Bovine Betacellulin |
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| ARU100 | GroPep | 100 µg | EUR 346 |
 Betacellulin antibody |
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| 70R-BR013 | Fitzgerald | 50 ug | EUR 273 |
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Description: Affinity purified Rabbit polyclonal Betacellulin antibody |
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 Betacellulin, Mouse |
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| HY-P7329 | MedChemExpress | 50ug | EUR 612 |
 Protein) Human Betacellulin (bTC) Protein |
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| 20-abx065524 | Abbexa |
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， Human) Betacellulin (HEK293-expressed)， Human |
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| HY-P7328 | MedChemExpress | 10ug | EUR 234 |
 Recombinant Human Betacellulin Protein |
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| PROTP35070-2 | BosterBio | 20ug | EUR 317 |
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Description: Betacellulin is an EGF-related polypeptide growth factor that signals through the EGF receptor. It is produced in several tissues, including the pancreas, small intestine, and in certain tumor cells. Betacellulin is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. Human Betacellulin is initially synthesized as a glycosylated 32.0 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce the mature sequence. Recombinant human Betacellulin is a 9.0 kDa monomeric protein, containing 80 amino residues, which comprises the mature EGF homologous portion of the Betacellulin protein. |
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 Polyclonal Goat anti-GST α-form |
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| GST-ANTI-1 | Detroit R&D | 50 uL | EUR 280 |
 Polyclonal Goat anti-GST μ-form |
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| GST-ANTI-2 | Detroit R&D | 50 uL | EUR 280 |
 Polyclonal Goat anti-GST p-form |
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| GST-ANTI-3 | Detroit R&D | 50 uL | EUR 280 |
 Recombinant Bovine Betacellulin |
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| 7-00226 | CHI Scientific | 5µg | Ask for price |
Recombinant Bovine Betacellulin |
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| 7-00227 | CHI Scientific | 20µg | Ask for price |
Recombinant Bovine Betacellulin |
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| 7-00228 | CHI Scientific | 1mg | Ask for price |
 Betacellulin, Murine Recombinant |
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| 7119-10 | Biovision | EUR 196 | |
Betacellulin, Murine Recombinant |
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| 7119-50 | Biovision | EUR 675 | |
 Antibody) Betacellulin (bTC) Antibody |
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| 20-abx175568 | Abbexa |
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Betacellulin (bTC) Antibody |
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| 20-abx175569 | Abbexa |
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Betacellulin (bTC) Antibody |
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| 20-abx175570 | Abbexa |
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Betacellulin (bTC) Antibody |
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| 20-abx175571 | Abbexa |
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Betacellulin (bTC) Antibody |
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| 20-abx100739 | Abbexa |
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Betacellulin (bTC) Antibody |
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| 20-abx100740 | Abbexa |
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 Antibody) Betacellulin (BTC) Antibody |
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| 20-abx111218 | Abbexa |
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Betacellulin (bTC) Antibody |
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| 20-abx171411 | Abbexa |
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Betacellulin (BTC) Antibody |
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| abx230883-100ug | Abbexa | 100 ug | EUR 481 |
) Betacellulin, murine (mouse) |
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| RC238-23 | Bio Basic | 5ug | EUR 104.38 |
) Recombinant Betacellulin (bTC) |
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| 4-RPA112Hu01 | Cloud-Clone |
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Description: Recombinant Human Betacellulin expressed in: E.coli |
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Recombinant Betacellulin (bTC) |
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| 4-RPA112Mu01 | Cloud-Clone |
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Description: Recombinant Mouse Betacellulin expressed in: E.coli |
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 ELISA Kit) Human Betacellulin (bTC) ELISA Kit |
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| DLR-bTC-Hu-48T | DL Develop | 48T | EUR 479 |
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Description: A sandwich quantitative ELISA assay kit for detection of Human Betacellulin (bTC) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids. |
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Human Betacellulin (bTC) ELISA Kit |
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| DLR-bTC-Hu-96T | DL Develop | 96T | EUR 621 |
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Description: A sandwich quantitative ELISA assay kit for detection of Human Betacellulin (bTC) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids. |
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 Human Betacellulin, BTC ELISA Kit |
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| DEIA089 | Creative Diagnostics | 10 plates | EUR 2605 |
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Description: This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). It is developed for quantitative measurement of human BTC in serum, plasma and other biological fluids. |
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 CLIA Kit) Human Betacellulin (bTC) CLIA Kit |
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| abx196482-96tests | Abbexa | 96 tests | EUR 825 |
 ELISA Kit) Human Betacellulin (BTC) ELISA Kit |
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| abx250182-96tests | Abbexa | 96 tests | EUR 668 |
 ELISA Kit) Human BTC(Betacellulin) ELISA Kit |
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| EH0044 | FN Test | 96T | EUR 524.1 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml |
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Human Betacellulin (BTC) ELISA Kit |
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| abx570040-96tests | Abbexa | 96 tests | EUR 668 |
 Protein (Active)) Human Betacellulin (bTC) Protein (Active) |
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| 20-abx655563 | Abbexa |
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 CLIA Kit) Human Betacellulin (BTC) CLIA Kit |
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| 20-abx491043 | Abbexa |
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 Human Betacellulin/BTC ELISA Kit |
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| LF-EK50996 | Abfrontier | 1×96T | EUR 648 |
 Polyclonal Antibody (Human)) Betacellulin (bTC) Polyclonal Antibody (Human) |
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| 4-PAA112Hu01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC) |
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 BTC Betacellulin Human Recombinant Protein |
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| PROTP35070-1 | BosterBio | Regular: 20ug | EUR 317 |
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Description: Betacellulin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 80 amino acids and having a molecular mass of 9 kDa. ;Betacellulin Human Recombinant is purified by proprietary chromatographic techniques. |
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Human Betacellulin (bTC) ELISA Kit |
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| RDR-bTC-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 500 |
Human Betacellulin (bTC) ELISA Kit |
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| RDR-bTC-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 692 |
Human Betacellulin (bTC) ELISA Kit |
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| SEA112Hu-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 4273.35 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids. |
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Human Betacellulin (bTC) ELISA Kit |
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| SEA112Hu-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 439.57 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids. |
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Human Betacellulin (bTC) ELISA Kit |
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| SEA112Hu-1x96wellstestplate | Cloud-Clone | 1x96-wells test plate | EUR 585.1 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids. |
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Human Betacellulin (bTC) ELISA Kit |
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| SEA112Hu-5x96wellstestplate | Cloud-Clone | 5x96-wells test plate | EUR 2332.95 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids. |
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Human Betacellulin (bTC) ELISA Kit |
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| 4-SEA112Hu | Cloud-Clone |
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Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Betacellulin (bTC) in samples from Serum, plasma, tissue homogenates, cell lysates and other biological fluids. with no significant corss-reactivity with analogues from other species. |
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Human Betacellulin (bTC) ELISA Kit |
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| RD-bTC-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 478 |
Human Betacellulin (bTC) ELISA Kit |
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| RD-bTC-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 662 |
 Protein) Mouse Betacellulin (bTC) Protein |
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| 20-abx065525 | Abbexa |
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 Antibody (Biotin)) Betacellulin (bTC) Antibody (Biotin) |
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| 20-abx271925 | Abbexa |
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Betacellulin (bTC) Antibody (Biotin) |
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| 20-abx272658 | Abbexa |
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 Protein) Rat Betacellulin (bTC) Protein |
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| 20-abx652663 | Abbexa |
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 Protein) Cow Betacellulin (bTC) Protein |
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| 20-abx652665 | Abbexa |
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 Protein) Chicken Betacellulin (bTC) Protein |
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| 20-abx652666 | Abbexa |
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 Antibody (FITC)) Betacellulin (bTC) Antibody (FITC) |
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| 20-abx274036 | Abbexa |
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, Mouse) Betacellulin (HEK293-expressed), Mouse |
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| HY-P7142 | MedChemExpress | 50ug | EUR 533 |
) Rat Betacellulin antiserum (Rabbit) |
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| PAAQ1 | GroPep | 100 µl | EUR 299 |
) Bovine Betacellulin antiserum (Rabbit) |
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| PAAR1 | GroPep | 100 µl | EUR 299 |
 Recombinant Murine Betacellulin Protein |
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| PROTQ05928-1 | BosterBio | 20ug | EUR 317 |
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Description: Betacellulin is an EGF-related polypeptide growth factor that signals through the EGF receptor. It is produced in several tissues, including the pancreas, small intestine, and in certain tumor cells. Betacellulin is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. Betacellulin is initially synthesized as a glycosylated 32.0 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce the mature sequence. Recombinant murine Betacellulin is a 9.0 kDa monomeric protein, containing 80 amino residues, which comprises the mature EGF homologous portion of the Betacellulin protein. |
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) CLIA kit for Human BTC (Betacellulin) |
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| E-CL-H0444 | Elabscience Biotech | 1 plate of 96 wells | EUR 584 |
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Description: A sandwich CLIA kit for quantitative measurement of Human BTC (Betacellulin) in samples from Serum, Plasma, Cell supernatant |
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) ELISA kit for Human BTC (Betacellulin) |
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| E-EL-H0570 | Elabscience Biotech | 1 plate of 96 wells | EUR 534 |
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Description: A sandwich ELISA kit for quantitative measurement of Human BTC (Betacellulin) in samples from Serum, Plasma, Cell supernatant |
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 ELISA kit for Human Betacellulin/BTC |
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| EK5454 | SAB | 96 tests | EUR 553 |
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Description: Enzyme-linked immunosorbent assay kit for quantification of Human Betacellulin/BTC in samples from serum, plasma, tissue homogenates and other biological fluids. |
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 Human Betacellulin/BTC PicoKine ELISA Kit |
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| EK0980 | BosterBio | 96 wells | EUR 425 |
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Description: For quantitative detection of human Betacellulin in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
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) ELISA kit for Human bTC (Betacellulin) |
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| ELK1403 | ELK Biotech | 1 plate of 96 wells | EUR 432 |
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Description: A sandwich ELISA kit for detection of Betacellulin from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
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 Polyclonal Antibody (Human), APC) Betacellulin (bTC) Polyclonal Antibody (Human), APC |
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| 4-PAA112Hu01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC). This antibody is labeled with APC. |
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 Polyclonal Antibody (Human), Biotinylated) Betacellulin (bTC) Polyclonal Antibody (Human), Biotinylated |
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| 4-PAA112Hu01-Biotin | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC). This antibody is labeled with Biotin. |
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 Polyclonal Antibody (Human), Cy3) Betacellulin (bTC) Polyclonal Antibody (Human), Cy3 |
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| 4-PAA112Hu01-Cy3 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC). This antibody is labeled with Cy3. |
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 Polyclonal Antibody (Human), FITC) Betacellulin (bTC) Polyclonal Antibody (Human), FITC |
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| 4-PAA112Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC). This antibody is labeled with FITC. |
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 Polyclonal Antibody (Human), HRP) Betacellulin (bTC) Polyclonal Antibody (Human), HRP |
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| 4-PAA112Hu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC). This antibody is labeled with HRP. |
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 Polyclonal Antibody (Human), PE) Betacellulin (bTC) Polyclonal Antibody (Human), PE |
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| 4-PAA112Hu01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC). This antibody is labeled with PE. |
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) Human Probetacellulin [Cleaved into: Betacellulin protein (BTC) |
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| 1-CSB-EP002853HU | Cusabio |
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Description: Recombinant Human Probetacellulin [Cleaved into: Betacellulin protein(BTC) expressed in E.coli |
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 Polyclonal Antibody (Human), APC-Cy7) Betacellulin (bTC) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAA112Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC). This antibody is labeled with APC-Cy7. |
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 : 96 Wells (OKAG00006)) Betacellulin ELISA Kit (Human) : 96 Wells (OKAG00006) |
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| OKAG00006 | Aviva Systems Biology | 96 Wells | EUR 596 |
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Description: Description of target: This gene encodes a member of the epidermal growth factor (EGF) family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the secreted growth factor. A secreted form and a membrane-anchored form of this protein bind to multiple different EGF receptors. This protein promotes pancreatic cell proliferation and insulin secretion, as well as retinal vascular permeability. Mutations in this gene may be associated with type 2 diabetes in human patients.;Species reactivity: Human;Application: ELISA;Assay info: Quantitative Colorimentric Sandwich ELISA;Sensitivity: 8 pg/mL |
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 BTC Betacellulin Human Recombinant Protein, His Tag |
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| PROTP35070 | BosterBio | Regular: 10ug | EUR 317 |
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Description: BTC Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 101 amino acids (32-111) and having a molecular mass of 11.3 kDa.;BTC is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
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 ELISA Kit) Mouse Betacellulin (bTC) ELISA Kit |
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| DLR-bTC-Mu-48T | DL Develop | 48T | EUR 433 |
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Description: A sandwich quantitative ELISA assay kit for detection of Mouse Betacellulin (bTC) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids. |
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Mouse Betacellulin (bTC) ELISA Kit |
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| DLR-bTC-Mu-96T | DL Develop | 96T | EUR 558 |
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Description: A sandwich quantitative ELISA assay kit for detection of Mouse Betacellulin (bTC) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids. |
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 ELISA Kit) Rat Betacellulin (bTC) ELISA Kit |
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| DLR-bTC-Ra-48T | DL Develop | 48T | EUR 508 |
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Description: A sandwich quantitative ELISA assay kit for detection of Rat Betacellulin (bTC) in samples from serum, plasma, tissue homogenates, cell lysates,cell culture supernates or other biological fluids. |
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Rat Betacellulin (bTC) ELISA Kit |
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| DLR-bTC-Ra-96T | DL Develop | 96T | EUR 661 |
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Description: A sandwich quantitative ELISA assay kit for detection of Rat Betacellulin (bTC) in samples from serum, plasma, tissue homogenates, cell lysates,cell culture supernates or other biological fluids. |
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 ELISA Kit) Mouse Betacellulin (BTC) ELISA Kit |
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| 20-abx153708 | Abbexa |
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Mouse Betacellulin (BTC) ELISA Kit |
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| abx253974-96tests | Abbexa | 96 tests | EUR 668 |
 ELISA Kit) Rat Betacellulin (BTC) ELISA Kit |
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| abx255590-96tests | Abbexa | 96 tests | EUR 668 |
 ELISA Kit) Monkey Betacellulin (BTC) ELISA Kit |
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| abx359865-96tests | Abbexa | 96 tests | EUR 825 |
 ELISA Kit) Pig Betacellulin (BTC) ELISA Kit |
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| abx361663-96tests | Abbexa | 96 tests | EUR 825 |
 ELISA Kit) Rabbit Betacellulin (BTC) ELISA Kit |
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| abx363832-96tests | Abbexa | 96 tests | EUR 825 |
 ELISA Kit) Chicken Betacellulin (BTC) ELISA Kit |
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| abx574267-96tests | Abbexa | 96 tests | EUR 825 |
